3l2p

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[[Image:3l2p.png|left|200px]]
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==Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States==
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<StructureSection load='3l2p' size='340' side='right' caption='[[3l2p]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3l2p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L2P FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LIG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l2p RCSB], [http://www.ebi.ac.uk/pdbsum/3l2p PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/3l2p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases DNA nick-joining and intermolecular DNA ligation. Yet, the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small angle x-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) form a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) -OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick-sensing and the transition from nick-sensing by the ZnF to nick-joining by the catalytic core. The collective results support a "jackknife model" whereby the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.
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{{STRUCTURE_3l2p| PDB=3l2p | SCENE= }}
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Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States.,Cotner-Gohara E, Kim IK, Hammel M, Tainer JA, Tomkinson AE, Ellenberger T Biochemistry. 2010 Jun 2. PMID:20518483<ref>PMID:20518483</ref>
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===Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20518483}}
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==About this Structure==
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[[3l2p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2P OCA].
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==See Also==
==See Also==
*[[DNA ligase|DNA ligase]]
*[[DNA ligase|DNA ligase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020518483</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Cotner-Gohara, E A.]]
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[[Category: Cotner-Gohara, E A]]
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[[Category: Ellenberger, T.]]
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[[Category: Ellenberger, T]]
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[[Category: Hammel, M.]]
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[[Category: Hammel, M]]
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[[Category: Kim, I K.]]
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[[Category: Kim, I K]]
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[[Category: Tainer, J A.]]
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[[Category: Tainer, J A]]
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[[Category: Tomkinson, A.]]
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[[Category: Tomkinson, A]]
[[Category: Atp-binding]]
[[Category: Atp-binding]]
[[Category: Cell cycle]]
[[Category: Cell cycle]]

Revision as of 08:52, 9 December 2014

Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States

3l2p, resolution 3.00Å

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