1mzj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1mzj.jpg|left|200px]]<br /><applet load="1mzj" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1mzj.jpg|left|200px]]
-
caption="1mzj, resolution 2.1&Aring;" />
+
 
-
'''Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway'''<br />
+
{{Structure
 +
|PDB= 1mzj |SIZE=350|CAPTION= <scene name='initialview01'>1mzj</scene>, resolution 2.1&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene> and <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41]
 +
|GENE= zhuH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=152628 Streptomyces sp. R22])
 +
}}
 +
 
 +
'''Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1MZJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r22 Streptomyces sp. r22] with <scene name='pdbligand=COA:'>COA</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZJ OCA].
+
1MZJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r22 Streptomyces sp. r22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZJ OCA].
==Reference==
==Reference==
-
Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway., Pan H, Tsai S, Meadows ES, Miercke LJ, Keatinge-Clay AT, O'Connell J, Khosla C, Stroud RM, Structure. 2002 Nov;10(11):1559-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12429097 12429097]
+
Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway., Pan H, Tsai S, Meadows ES, Miercke LJ, Keatinge-Clay AT, O'Connell J, Khosla C, Stroud RM, Structure. 2002 Nov;10(11):1559-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12429097 12429097]
[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 29: Line 38:
[[Category: catalytic triad]]
[[Category: catalytic triad]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:43 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:50:08 2008''

Revision as of 10:50, 20 March 2008

Image:1mzj.jpg


PDB ID 1mzj

Drag the structure with the mouse to rotate
, resolution 2.1Å
Ligands: and
Gene: zhuH (Streptomyces sp. R22)
Activity: Beta-ketoacyl-acyl-carrier-protein synthase I, with EC number 2.3.1.41
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the Priming beta-Ketosynthase from the R1128 Polyketide Biosynthetic Pathway


Overview

ZhuH is a priming ketosynthase that initiates the elongation of the polyketide chain in the biosynthetic pathway of a type II polyketide, R1128. The crystal structure of ZhuH in complex with the priming substrate acetyl-CoA reveals an extensive loop region at the dimer interface that appears to affect the selectivity for the primer unit. Acetyl-CoA is bound in a 20 A-long channel, which placed the acetyl group against the catalytic triad. Analysis of the primer unit binding site in ZhuH suggests that it can accommodate acyl chains that are two to four carbons long. Selectivity and primer unit size appear to involve the side chains of three residues on the loops close to the dimer interface that constitute the bottom of the substrate binding pocket.

About this Structure

1MZJ is a Single protein structure of sequence from Streptomyces sp. r22. Full crystallographic information is available from OCA.

Reference

Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway., Pan H, Tsai S, Meadows ES, Miercke LJ, Keatinge-Clay AT, O'Connell J, Khosla C, Stroud RM, Structure. 2002 Nov;10(11):1559-68. PMID:12429097

Page seeded by OCA on Thu Mar 20 12:50:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mzj"
Personal tools