3n3k

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[[Image:3n3k.png|left|200px]]
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==The catalytic domain of USP8 in complex with a USP8 specific inhibitor==
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<StructureSection load='3n3k' size='340' side='right' caption='[[3n3k]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3n3k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N3K FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gfo|2gfo]], [[2a9u|2a9u]], [[1whb|1whb]], [[2fzp|2fzp]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIAA0055, UBPY, USP8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, UBQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n3k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n3k RCSB], [http://www.ebi.ac.uk/pdbsum/3n3k PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/3n3k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ubiquitin system regulates virtually all aspects of cellular function. We report a method to target the myriad enzymes that govern ubiquitination of protein substrates. We used massively diverse combinatorial libraries of ubiquitin variants to develop inhibitors of four deubiquitinases (DUBs) and analyzed the DUB-inhibitor complexes with crystallography. We extended the selection strategy to the ubiquitin conjugating (E2) and ubiquitin ligase (E3) enzymes and found that ubiquitin variants can also enhance enzyme activity. Last, we showed that ubiquitin variants can bind selectively to ubiquitin-binding domains. Ubiquitin variants exhibit selective function in cells and thus enable orthogonal modulation of specific enzymatic steps in the ubiquitin system.
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{{STRUCTURE_3n3k| PDB=3n3k | SCENE= }}
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A strategy for modulation of enzymes in the ubiquitin system.,Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai AM, Neculai D, Vorobyov A, Garg P, Beatty L, Chan PK, Juang YC, Landry MC, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedadi M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, Rotin D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS Science. 2013 Feb 1;339(6119):590-5. doi: 10.1126/science.1230161. Epub 2013 Jan , 3. PMID:23287719<ref>PMID:23287719</ref>
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===The catalytic domain of USP8 in complex with a USP8 specific inhibitor===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_23287719}}
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==About this Structure==
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[[3n3k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N3K OCA].
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==See Also==
==See Also==
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*[[Thioesterase|Thioesterase]]
*[[Ubiquitin|Ubiquitin]]
*[[Ubiquitin|Ubiquitin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017035239</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Ubiquitin thiolesterase]]
[[Category: Ubiquitin thiolesterase]]
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[[Category: Allali-Hassani, A.]]
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[[Category: Allali-Hassani, A]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Arrowsmith, C H]]
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[[Category: Avvakumov, G V.]]
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[[Category: Avvakumov, G V]]
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[[Category: Bochkarev, A.]]
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[[Category: Bochkarev, A]]
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[[Category: Bountra, C.]]
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[[Category: Bountra, C]]
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[[Category: Consortium, Structural Genomics.]]
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[[Category: Structural genomic]]
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[[Category: Dhe-Paganon, S.]]
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[[Category: Dhe-Paganon, S]]
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[[Category: Edwards, A M.]]
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[[Category: Edwards, A M]]
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[[Category: Ernst, A.]]
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[[Category: Ernst, A]]
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[[Category: Lam, R.]]
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[[Category: Lam, R]]
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[[Category: Li, Y.]]
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[[Category: Li, Y]]
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[[Category: Sidhu, S.]]
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[[Category: Sidhu, S]]
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[[Category: Walker, J R.]]
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[[Category: Walker, J R]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt, J]]
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[[Category: Xue, S.]]
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[[Category: Xue, S]]
[[Category: Deubiquitinating enzyme]]
[[Category: Deubiquitinating enzyme]]
[[Category: Dub]]
[[Category: Dub]]
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[[Category: Protease]]
[[Category: Protease]]
[[Category: Sgc]]
[[Category: Sgc]]
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[[Category: Structural genomic]]
 
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[[Category: Structural genomics consortium]]
 
[[Category: Thiol protease]]
[[Category: Thiol protease]]
[[Category: Ubiquitin]]
[[Category: Ubiquitin]]
[[Category: Ubl conjugation pathway]]
[[Category: Ubl conjugation pathway]]
[[Category: Zinc ribbon]]
[[Category: Zinc ribbon]]

Revision as of 09:59, 9 December 2014

The catalytic domain of USP8 in complex with a USP8 specific inhibitor

3n3k, resolution 2.60Å

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