3m4d

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[[Image:3m4d.png|left|200px]]
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==Crystal structure of the M113N mutant of alpha-hemolysin==
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<StructureSection load='3m4d' size='340' side='right' caption='[[3m4d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3m4d]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M4D FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m3r|3m3r]], [[3m4e|3m4e]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hla, hly ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m4d RCSB], [http://www.ebi.ac.uk/pdbsum/3m4d PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/3m4d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and micro-devices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the alpha-hemolysin (alphaHL) pore that bind the adapter beta-cyclodextrin (betaCD) approximately 10(4) times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanopores in unparalleled detail.
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{{STRUCTURE_3m4d| PDB=3m4d | SCENE= }}
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Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.,Banerjee A, Mikhailova E, Cheley S, Gu LQ, Montoya M, Nagaoka Y, Gouaux E, Bayley H Proc Natl Acad Sci U S A. 2010 Apr 16. PMID:20400691<ref>PMID:20400691</ref>
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===Crystal structure of the M113N mutant of alpha-hemolysin===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20400691}}
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==About this Structure==
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[[3m4d]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M4D OCA].
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==See Also==
==See Also==
*[[Hemolysin|Hemolysin]]
*[[Hemolysin|Hemolysin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020400691</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Gouaux, E.]]
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[[Category: Gouaux, E]]
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[[Category: Montoya, M.]]
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[[Category: Montoya, M]]
[[Category: Beta-barrel]]
[[Category: Beta-barrel]]
[[Category: Cell invasion]]
[[Category: Cell invasion]]

Revision as of 10:00, 9 December 2014

Crystal structure of the M113N mutant of alpha-hemolysin

3m4d, resolution 1.90Å

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