3qe4
From Proteopedia
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| - | [[ | + | ==An evolved aminoacyl-tRNA Synthetase with atypical polysubstrate specificity== |
| + | <StructureSection load='3qe4' size='340' side='right' caption='[[3qe4]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3qe4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QE4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4CF:4-CYANO-L-PHENYLALANINE'>4CF</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ag6|2ag6]], [[1zh0|1zh0]], [[1zh6|1zh6]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MJ0389, TyrRS, tyrS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qe4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qe4 RCSB], [http://www.ebi.ac.uk/pdbsum/3qe4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aminoacyl-tRNA synthetases (aaRSs) against an array of unnatural amino acids. We discovered that a p-cyanophenylalanine specific aminoacyl-tRNA synthetase (pCNF-RS) has high substrate permissivity for unnatural amino acids, while maintaining its ability to discriminate against the 20 canonical amino acids. This orthogonal pCNF-RS, together with its cognate amber nonsense suppressor tRNA, is able to selectively incorporate 18 unnatural amino acids into proteins, including trifluoroketone-, alkynyl-, and halogen-substituted amino acids. In an attempt to improve our understanding of this polyspecificity, the X-ray crystal structure of the aaRS-p-cyanophenylalanine complex was determined. A comparison of this structure with those of other mutant aaRSs showed that both binding site size and other more subtle features control substrate polyspecificity. | ||
| - | + | An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity .,Young DD, Young TS, Jahnz M, Ahmad I, Spraggon G, Schultz PG Biochemistry. 2011 Feb 1. PMID:21280675<ref>PMID:21280675</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]] | *[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Tyrosine--tRNA ligase]] | [[Category: Tyrosine--tRNA ligase]] | ||
| - | [[Category: Ahmad, I | + | [[Category: Ahmad, I]] |
| - | [[Category: Jahnz, M | + | [[Category: Jahnz, M]] |
| - | [[Category: Schultz, P G | + | [[Category: Schultz, P G]] |
| - | [[Category: Spraggon, G | + | [[Category: Spraggon, G]] |
| - | [[Category: Young, D D | + | [[Category: Young, D D]] |
| - | [[Category: Young, T S | + | [[Category: Young, T S]] |
[[Category: Evolved trna synthetase]] | [[Category: Evolved trna synthetase]] | ||
[[Category: Ligase]] | [[Category: Ligase]] | ||
[[Category: Trna]] | [[Category: Trna]] | ||
[[Category: Trna synthetase evolved to bind unnatural amino acid]] | [[Category: Trna synthetase evolved to bind unnatural amino acid]] | ||
Revision as of 11:28, 9 December 2014
An evolved aminoacyl-tRNA Synthetase with atypical polysubstrate specificity
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