3r6r
From Proteopedia
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| - | [[ | + | ==Structure of the complex of an intramolecular human telomeric DNA with Berberine formed in K+ solution== |
| + | <StructureSection load='3r6r' size='340' side='right' caption='[[3r6r]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3r6r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R6R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R6R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BER:BERBERINE'>BER</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r6r RCSB], [http://www.ebi.ac.uk/pdbsum/3r6r PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The first crystal structure of human telomeric DNA in complex with the natural alkaloid berberine, produced by different plant families and used in folk medicine for millennia, was solved by X-ray diffraction method. The G-quadruplex unit features all-parallel strands. The overall folding assumed by DNA is the same found in previously reported crystal structures. Similarly to previously reported structures the ligand molecules were found to be stacked onto the external 5' and 3'-end G-tetrads. However, the present crystal structure highlighted for the first time, the presence of two berberine molecules in the two binding sites, directly interacting with each tetrad. As a consequence, our structural data point out a 2:1 ligand to G-tetrad molar ratio, which has never been reported before in a telomeric intramolecular quadruplex structure. | ||
| - | + | The crystal structure of human telomeric DNA complexed with berberine: an interesting case of stacked ligand to G-tetrad ratio higher than 1:1.,Bazzicalupi C, Ferraroni M, Bilia AR, Scheggi F, Gratteri P Nucleic Acids Res. 2013 Jan 1;41(1):632-8. doi: 10.1093/nar/gks1001. Epub 2012, Oct 26. PMID:23104378<ref>PMID:23104378</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
| - | [[Category: Bazzicalupi, C | + | [[Category: Bazzicalupi, C]] |
| - | [[Category: Bilia, A R | + | [[Category: Bilia, A R]] |
| - | [[Category: Ferraroni, M | + | [[Category: Ferraroni, M]] |
| - | [[Category: Gratteri, P | + | [[Category: Gratteri, P]] |
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Dna-antibiotic complex]] | [[Category: Dna-antibiotic complex]] | ||
Revision as of 11:36, 9 December 2014
Structure of the complex of an intramolecular human telomeric DNA with Berberine formed in K+ solution
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