1nbw
From Proteopedia
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| - | [[Image:1nbw.jpg|left|200px]] | + | [[Image:1nbw.jpg|left|200px]] |
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| - | '''Glycerol dehydratase reactivase''' | + | {{Structure |
| + | |PDB= 1nbw |SIZE=350|CAPTION= <scene name='initialview01'>1nbw</scene>, resolution 2.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= dhaB4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]), orf2b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]) | ||
| + | }} | ||
| + | |||
| + | '''Glycerol dehydratase reactivase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NBW is a [ | + | 1NBW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBW OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of glycerol dehydratase reactivase: a new type of molecular chaperone., Liao DI, Reiss L, Turner I, Dotson G, Structure. 2003 Jan;11(1):109-19. PMID:[http:// | + | Structure of glycerol dehydratase reactivase: a new type of molecular chaperone., Liao DI, Reiss L, Turner I, Dotson G, Structure. 2003 Jan;11(1):109-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12517345 12517345] |
[[Category: Klebsiella pneumoniae]] | [[Category: Klebsiella pneumoniae]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: reactivase]] | [[Category: reactivase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:54:48 2008'' |
Revision as of 10:54, 20 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | dhaB4 (Klebsiella pneumoniae), orf2b (Klebsiella pneumoniae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Glycerol dehydratase reactivase
Overview
The function of glycerol dehydratase (GDH) reactivase is to remove damaged coenzyme B(12) from GDH that has suffered mechanism-based inactivation. The structure of GDH reactivase from Klebsiella pneumoniae was determined at 2.4 A resolution by the single isomorphous replacement with anomalous signal (SIR/AS) method. Each tetramer contains two elongated 63 kDa alpha subunits and two globular 14 kDa beta subunits. The alpha subunit contains structural features resembling both GroEL and Hsp70 groups of chaperones, and it appears chaperone like in its interactions with ATP. The fold of the beta subunit resembles that of the beta subunit of glycerol dehydratase, except that it lacks some coenzyme B(12) binding elements. A hypothesis for the reactivation mechanism of reactivase is proposed based on these structural features.
About this Structure
1NBW is a Protein complex structure of sequences from Klebsiella pneumoniae. Full crystallographic information is available from OCA.
Reference
Structure of glycerol dehydratase reactivase: a new type of molecular chaperone., Liao DI, Reiss L, Turner I, Dotson G, Structure. 2003 Jan;11(1):109-19. PMID:12517345
Page seeded by OCA on Thu Mar 20 12:54:48 2008
