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Publication Abstract from PubMed

7,8-dihydro-8-oxoguanine (8-oxoG) adducts are formed frequently by the attack of oxygen-free radicals on DNA. They are among the most mutagenic lesions in cells because of their dual coding potential, where, in addition to normal base-pairing of 8-oxoG(anti) with dCTP, 8-oxoG in the syn conformation can base pair with dATP, causing G to T transversions. We provide here for the first time a structural basis for the error-free replication of 8-oxoG lesions by yeast DNA polymerase eta (Poleta). We show that the open active site cleft of Poleta can accommodate an 8-oxoG lesion in the anti conformation with only minimal changes to the polymerase and the bound DNA: at both the insertion and post-insertion steps of lesion bypass. Importantly, the active site geometry remains the same as in the undamaged complex and provides a basis for the ability of Poleta to prevent the mutagenic replication of 8-oxoG lesions in cells.

Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase eta.,Silverstein TD, Jain R, Johnson RE, Prakash L, Prakash S, Aggarwal AK Structure. 2010 Nov 10;18(11):1463-70. PMID:21070945^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.