3pjs
From Proteopedia
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| - | [[ | + | ==Mechanism of Activation Gating in the Full-Length KcsA K+ Channel== |
| + | <StructureSection load='3pjs' size='340' side='right' caption='[[3pjs]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3pjs]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PJS FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">kcsA, skc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1916 Streptomyces lividans])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pjs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pjs RCSB], [http://www.ebi.ac.uk/pdbsum/3pjs PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Using a constitutively active channel mutant, we solved the structure of full-length KcsA in the open conformation at 3.9 A. The structure reveals that the activation gate expands about 20 A, exerting a strain on the bulge helices in the C-terminal domain and generating side windows large enough to accommodate hydrated K(+) ions. Functional and spectroscopic analysis of the gating transition provides direct insight into the allosteric coupling between the activation gate and the selectivity filter. We show that the movement of the inner gate helix is transmitted to the C-terminus as a straightforward expansion, leading to an upward movement and the insertion of the top third of the bulge helix into the membrane. We suggest that by limiting the extent to which the inner gate can open, the cytoplasmic domain also modulates the level of inactivation occurring at the selectivity filter. | ||
| - | + | Mechanism of activation gating in the full-length KcsA K+ channel.,Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E Proc Natl Acad Sci U S A. 2011 Jul 5. PMID:21730186<ref>PMID:21730186</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Antibody|Antibody]] | *[[Antibody|Antibody]] | ||
| - | + | *[[Potassium Channel|Potassium Channel]] | |
| - | == | + | == References == |
| - | < | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Streptomyces lividans]] | [[Category: Streptomyces lividans]] | ||
| - | [[Category: Cuello, L G | + | [[Category: Cuello, L G]] |
| - | [[Category: Kossiakoff, A | + | [[Category: Kossiakoff, A]] |
| - | [[Category: Perozo, E | + | [[Category: Perozo, E]] |
| - | [[Category: Uysal, S | + | [[Category: Uysal, S]] |
[[Category: Cell membrane]] | [[Category: Cell membrane]] | ||
[[Category: Conducts k+ ion]] | [[Category: Conducts k+ ion]] | ||
[[Category: Ion channel]] | [[Category: Ion channel]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 12:06, 9 December 2014
Mechanism of Activation Gating in the Full-Length KcsA K+ Channel
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