1nhl
From Proteopedia
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| - | [[Image:1nhl.jpg|left|200px]] | + | [[Image:1nhl.jpg|left|200px]] |
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| - | '''SNAP-23N Structure''' | + | {{Structure |
| + | |PDB= 1nhl |SIZE=350|CAPTION= <scene name='initialview01'>1nhl</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SNAP23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''SNAP-23N Structure''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NHL is a [ | + | 1NHL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHL OCA]. |
==Reference== | ==Reference== | ||
| - | Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:[http:// | + | Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12556468 12556468] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: snare]] | [[Category: snare]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:00 2008'' |
Revision as of 10:57, 20 March 2008
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| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | SNAP23 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SNAP-23N Structure
Overview
SNARE proteins mediate intracellular membrane fusion by forming a coiled-coil complex to merge opposing membranes. A "fusion-active" neuronal SNARE complex is a parallel four-helix bundle containing two coiled-coil domains from SNAP-25 and one coiled-coil domain each from syntaxin-1a and VAMP-2. "Prefusion" assembly intermediate complexes can also form from these SNAREs. We studied the N-terminal coiled-coil domain of SNAP-23 (SNAP-23N), a non-neuronal homologue of SNAP-25, and its interaction with other coiled-coil domains. SNAP-23N can assemble spontaneously with the coiled-coil domains from SNAP-23C, syntaxin-4, and VAMP-3 to form a heterotetrameric complex. Unexpectedly, pure SNAP-23N crystallizes as a coiled-coil homotetrameric complex. The four helices have a parallel orientation and are symmetrical about the long axis. The complex is stabilized through the interaction of conserved hydrophobic residues comprising the a and d positions of the coiled-coil heptad repeats. In addition, a central, highly conserved glutamine residue (Gln-48) is buried within the interface by hydrogen bonding between glutamine side chains derived from adjacent subunits and to solvent molecules. A comparison of the SNAP-23N structure to other SNARE complex structures reveals how a simple coiled-coil motif can form diverse SNARE complexes.
About this Structure
1NHL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Homotetrameric structure of the SNAP-23 N-terminal coiled-coil domain., Freedman SJ, Song HK, Xu Y, Sun ZY, Eck MJ, J Biol Chem. 2003 Apr 11;278(15):13462-7. Epub 2003 Jan 29. PMID:12556468
Page seeded by OCA on Thu Mar 20 12:57:00 2008
Categories: Homo sapiens | Single protein | Eck, M J. | Freedman, S J. | Song, H K. | Xu, Y. | Coiled-coil | Snare
