3p56

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[[Image:3p56.png|left|200px]]
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==The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease==
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<StructureSection load='3p56' size='340' side='right' caption='[[3p56]], [[Resolution|resolution]] 4.06&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3p56]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P56 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P56 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3p5j|3p5j]], [[3kio|3kio]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RNASEH2A, RNASEHI, RNHIA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), DLEU8, RNASEH2B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), AYP1, RNASEH2C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p56 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p56 RCSB], [http://www.ebi.ac.uk/pdbsum/3p56 PDBsum]</span></td></tr>
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</table>
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== Disease ==
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[[http://www.uniprot.org/uniprot/RNH2A_HUMAN RNH2A_HUMAN]] Aicardi-Goutieres syndrome. The disease is caused by mutations affecting the gene represented in this entry. [[http://www.uniprot.org/uniprot/RNH2C_HUMAN RNH2C_HUMAN]] Aicardi-Goutieres syndrome. The disease is caused by mutations affecting the gene represented in this entry. [[http://www.uniprot.org/uniprot/RNH2B_HUMAN RNH2B_HUMAN]] Aicardi-Goutieres syndrome. The disease is caused by mutations affecting the gene represented in this entry.
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== Function ==
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[[http://www.uniprot.org/uniprot/RNH2A_HUMAN RNH2A_HUMAN]] Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.<ref>PMID:16845400</ref> <ref>PMID:21177858</ref> [[http://www.uniprot.org/uniprot/RNH2C_HUMAN RNH2C_HUMAN]] Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.<ref>PMID:16845400</ref> <ref>PMID:21177858</ref> [[http://www.uniprot.org/uniprot/RNH2B_HUMAN RNH2B_HUMAN]] Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.<ref>PMID:21177858</ref> <ref>PMID:16845400</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribonuclease H2 (RNase H2) is the major nuclear enzyme involved in the degradation of RNA/DNA hybrids and removal of ribonucleotides misincorporated in genomic DNA. Mutations in each of the three RNase H2 subunits have been implicated in a human auto-inflammatory disorder, Aicardi-Goutieres Syndrome (AGS). To understand how mutations impact on RNase H2 function we determined the crystal structure of the human heterotrimer. In doing so, we correct several key regions of the previously reported murine RNase H2 atomic model and provide biochemical validation for our structural model. Our results provide new insights into how the subunits are arranged to form an enzymatically active complex. In particular, we establish that the RNASEH2A C terminus is a eukaryotic adaptation for binding the two accessory subunits, with residues within it required for enzymatic activity. This C-terminal extension interacts with the RNASEH2C C terminus and both are necessary to form a stable, enzymatically active heterotrimer. Disease mutations cluster at this interface between all three subunits, destabilizing the complex and/or impairing enzyme activity. Altogether, we locate 25 out of 29 residues mutated in AGS patients, establishing a firm basis for future investigations into disease pathogenesis and function of the RNase H2 enzyme.
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{{STRUCTURE_3p56| PDB=3p56 | SCENE= }}
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The Structure of the Human RNase H2 Complex Defines Key Interaction Interfaces Relevant to Enzyme Function and Human Disease.,Reijns MA, Bubeck D, Gibson LC, Graham SC, Baillie GS, Jones EY, Jackson AP J Biol Chem. 2011 Mar 25;286(12):10530-9. Epub 2010 Dec 22. PMID:21177854<ref>PMID:21177854</ref>
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===The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_21177854}}
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==About this Structure==
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[[3p56]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P56 OCA].
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==See Also==
==See Also==
*[[Ribonuclease|Ribonuclease]]
*[[Ribonuclease|Ribonuclease]]
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*[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:021177854</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Ribonuclease H]]
[[Category: Ribonuclease H]]
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[[Category: Bubeck, D.]]
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[[Category: Bubeck, D]]
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[[Category: Graham, S C.]]
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[[Category: Graham, S C]]
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[[Category: Jones, E Y.]]
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[[Category: Jones, E Y]]
[[Category: Hydrolase-replication complex]]
[[Category: Hydrolase-replication complex]]
[[Category: Nuclease that cleaves rna/dna hybrid]]
[[Category: Nuclease that cleaves rna/dna hybrid]]

Revision as of 13:34, 9 December 2014

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease

3p56, resolution 4.06Å

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