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1nne

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[[Image:1nne.gif|left|200px]]<br /><applet load="1nne" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1nne.gif|left|200px]]
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caption="1nne, resolution 3.11&Aring;" />
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'''Crystal Structure of the MutS-ADPBeF3-DNA complex'''<br />
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{{Structure
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|PDB= 1nne |SIZE=350|CAPTION= <scene name='initialview01'>1nne</scene>, resolution 3.11&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure of the MutS-ADPBeF3-DNA complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NNE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=BEF:'>BEF</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNE OCA].
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1NNE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNE OCA].
==Reference==
==Reference==
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Crystal structure and biochemical analysis of the MutS.ADP.beryllium fluoride complex suggests a conserved mechanism for ATP interactions in mismatch repair., Alani E, Lee JY, Schofield MJ, Kijas AW, Hsieh P, Yang W, J Biol Chem. 2003 May 2;278(18):16088-94. Epub 2003 Feb 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12582174 12582174]
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Crystal structure and biochemical analysis of the MutS.ADP.beryllium fluoride complex suggests a conserved mechanism for ATP interactions in mismatch repair., Alani E, Lee JY, Schofield MJ, Kijas AW, Hsieh P, Yang W, J Biol Chem. 2003 May 2;278(18):16088-94. Epub 2003 Feb 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12582174 12582174]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: dna]]
[[Category: dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:07:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:59:10 2008''

Revision as of 10:59, 20 March 2008

Image:1nne.gif


PDB ID 1nne

Drag the structure with the mouse to rotate
, resolution 3.11Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the MutS-ADPBeF3-DNA complex


Overview

During mismatch repair ATP binding and hydrolysis activities by the MutS family proteins are important for both mismatch recognition and for transducing mismatch recognition signals to downstream repair factors. Despite intensive efforts, a MutS.ATP.DNA complex has eluded crystallographic analysis. Searching for ATP analogs that strongly bound to Thermus aquaticus (Taq) MutS, we found that ADP.beryllium fluoride (ABF), acted as a strong inhibitor of several MutS family ATPases. Furthermore, ABF promoted the formation of a ternary complex containing the Saccharomyces cerevisiae MSH2.MSH6 and MLH1.PMS1 proteins bound to mismatch DNA but did not promote dissociation of MSH2.MSH6 from mismatch DNA. Crystallographic analysis of the Taq MutS.DNA.ABF complex indicated that although this complex was very similar to that of MutS.DNA.ADP, both ADP.Mg(2+) moieties in the MutS. DNA.ADP structure were replaced by ABF. Furthermore, a disordered region near the ATP-binding pocket in the MutS B subunit became traceable, whereas the equivalent region in the A subunit that interacts with the mismatched nucleotide remained disordered. Finally, the DNA binding domains of MutS together with the mismatched DNA were shifted upon binding of ABF. We hypothesize that the presence of ABF is communicated between the two MutS subunits through the contact between the ordered loop and Domain III in addition to the intra-subunit helical lever arm that links the ATPase and DNA binding domains.

About this Structure

1NNE is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Crystal structure and biochemical analysis of the MutS.ADP.beryllium fluoride complex suggests a conserved mechanism for ATP interactions in mismatch repair., Alani E, Lee JY, Schofield MJ, Kijas AW, Hsieh P, Yang W, J Biol Chem. 2003 May 2;278(18):16088-94. Epub 2003 Feb 11. PMID:12582174

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