1noh
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1noh.gif|left|200px]] | + | [[Image:1noh.gif|left|200px]] |
| - | + | ||
| - | '''The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly''' | + | {{Structure |
| + | |PDB= 1noh |SIZE=350|CAPTION= <scene name='initialview01'>1noh</scene>, resolution 2.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= gp7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=221993 Vibrio phage f237]) | ||
| + | }} | ||
| + | |||
| + | '''The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1NOH is a [ | + | 1NOH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOH OCA]. |
==Reference== | ==Reference== | ||
| - | Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:[http:// | + | Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12778115 12778115] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio phage f237]] | [[Category: Vibrio phage f237]] | ||
| Line 23: | Line 32: | ||
[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:59:31 2008'' |
Revision as of 10:59, 20 March 2008
| |||||||
| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | gp7 (Vibrio phage f237) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of bacteriophage phi29 scaffolding protein gp7 after prohead assembly
Overview
Three-dimensional structures of the double-stranded DNA bacteriophage phi29 scaffolding protein (gp7) before and after prohead assembly have been determined at resolutions of 2.2 and 2.8 A, respectively. Both structures are dimers that resemble arrows, with a four-helix bundle composing the arrowhead and a coiled coil forming the tail. The structural resemblance of gp7 to the yeast transcription factor GCN4 suggests a DNA-binding function that was confirmed by native gel electrophoresis. DNA binding to gp7 may have a role in mediating the structural transition from prohead to mature virus and scaffold release. A cryo-EM analysis indicates that gp7 is arranged inside the capsid as a series of concentric shells. The position of the higher density features in these shells correlates with the positions of hexamers in the equatorial region of the capsid, suggesting that gp7 may regulate formation of the prolate head through interactions with these hexamers.
About this Structure
1NOH is a Single protein structure of sequence from Vibrio phage f237. Full crystallographic information is available from OCA.
Reference
Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:12778115
Page seeded by OCA on Thu Mar 20 12:59:31 2008
