1npb
From Proteopedia
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| - | [[Image:1npb.jpg|left|200px]] | + | [[Image:1npb.jpg|left|200px]] |
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| - | '''Crystal structure of the fosfomycin resistance protein from transposon Tn2921''' | + | {{Structure |
| + | |PDB= 1npb |SIZE=350|CAPTION= <scene name='initialview01'>1npb</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= TN2921 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=615 Serratia marcescens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the fosfomycin resistance protein from transposon Tn2921''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NPB is a [ | + | 1NPB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPB OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of fosfomycin resistance protein FosA from transposon Tn2921., Pakhomova S, Rife CL, Armstrong RN, Newcomer ME, Protein Sci. 2004 May;13(5):1260-5. Epub 2004 Apr 9. PMID:[http:// | + | Structure of fosfomycin resistance protein FosA from transposon Tn2921., Pakhomova S, Rife CL, Armstrong RN, Newcomer ME, Protein Sci. 2004 May;13(5):1260-5. Epub 2004 Apr 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15075406 15075406] |
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: potassium binding loop]] | [[Category: potassium binding loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:59:55 2008'' |
Revision as of 10:59, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | TN2921 (Serratia marcescens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the fosfomycin resistance protein from transposon Tn2921
Overview
The crystal structure of fosfomycin resistance protein FosA from transposon Tn2921 has been established at a resolution of 2.5 A. The protein crystallized without bound Mn(II) and K+, ions crucial for efficient catalysis, providing a structure of the apo enzyme. The protein maintains the three-dimensional domain-swapped arrangement of the paired betaalphabetabetabeta-motifs observed in the genomically encoded homologous enzyme from Pseudomonas aeruginosa (PA1129). The basic architecture of the active site is also maintained, despite the absence of the catalytically essential Mn(II). However, the absence of K+, which has been shown to enhance enzymatic activity, appears to contribute to conformational heterogeneity in the K(+)-binding loops.
About this Structure
1NPB is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Structure of fosfomycin resistance protein FosA from transposon Tn2921., Pakhomova S, Rife CL, Armstrong RN, Newcomer ME, Protein Sci. 2004 May;13(5):1260-5. Epub 2004 Apr 9. PMID:15075406
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