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1nq6
From Proteopedia
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| - | [[Image:1nq6.jpg|left|200px]] | + | [[Image:1nq6.jpg|left|200px]] |
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| - | '''Crystal Structure of the catalytic domain of xylanase A from Streptomyces halstedii JM8''' | + | {{Structure |
| + | |PDB= 1nq6 |SIZE=350|CAPTION= <scene name='initialview01'>1nq6</scene>, resolution 1.78Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] | ||
| + | |GENE= xysA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1944 Streptomyces halstedii]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the catalytic domain of xylanase A from Streptomyces halstedii JM8''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NQ6 is a [ | + | 1NQ6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_halstedii Streptomyces halstedii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NQ6 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of xylanase Xys1delta from Streptomyces halstedii., Canals A, Vega MC, Gomis-Ruth FX, Diaz M, Santamaria R RI, Coll M, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1447-53. Epub 2003, Jul 23. PMID:[http:// | + | Structure of xylanase Xys1delta from Streptomyces halstedii., Canals A, Vega MC, Gomis-Ruth FX, Diaz M, Santamaria R RI, Coll M, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1447-53. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876348 12876348] |
[[Category: Endo-1,4-beta-xylanase]] | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:00:16 2008'' |
Revision as of 11:00, 20 March 2008
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| , resolution 1.78Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | xysA (Streptomyces halstedii) | ||||||
| Activity: | Endo-1,4-beta-xylanase, with EC number 3.2.1.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the catalytic domain of xylanase A from Streptomyces halstedii JM8
Overview
Xylanases hydrolyze the beta-1,4-linked xylose backbone of xylans. They are of increasing interest in the paper and food industries for their pre-bleaching and bio-pulping applications. Such industries demand new xylanases to cover a wider range of cleavage specificity, activity and stability. The catalytic domain of xylanase Xys1 from Streptomyces halstedii JM8 was expressed, purified and crystallized and native data were collected to 1.78 A resolution with an R(merge) of 4.4%. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.05, b = 79.60, c = 87.80 A. The structure was solved by the molecular-replacement method using the structure of the homologue Xyl10A from Streptomyces lividans. In a similar manner to other members of its family, Xys1 folds to form a standard (beta/alpha)(8) barrel with the two catalytic functions, the acid/base and the nucleophile, at its C-terminal side. The overall structure is described and compared with those of related xylanases.
About this Structure
1NQ6 is a Single protein structure of sequence from Streptomyces halstedii. Full crystallographic information is available from OCA.
Reference
Structure of xylanase Xys1delta from Streptomyces halstedii., Canals A, Vega MC, Gomis-Ruth FX, Diaz M, Santamaria R RI, Coll M, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1447-53. Epub 2003, Jul 23. PMID:12876348
Page seeded by OCA on Thu Mar 20 13:00:16 2008
