4a7p
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea== |
| + | <StructureSection load='4a7p' size='340' side='right' caption='[[4a7p]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4a7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_elodea Sphingomonas elodea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A7P FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a7p RCSB], [http://www.ebi.ac.uk/pdbsum/4a7p PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gellan gum, a commercial gelling agent produced by Sphingomonas elodea ATCC 31461, is a high-value microbial exopolysaccharide. UDP-glucose dehydrogenase (UGD; EC 1.1.1.22) is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis. S. elodea ATCC 31461 UGD, termed UgdG, was cloned, expressed, purified and crystallized in native and SeMet-derivatized forms in hexagonal and tetragonal space groups, respectively; the crystals diffracted X-rays to 2.40 and 3.40 A resolution, respectively. Experimental phases were obtained for the tetragonal SeMet-derivatized crystal form by a single-wavelength anomalous dispersion experiment. This structure was successfully used as a molecular-replacement probe for the hexagonal crystal form of the native protein. | ||
| - | + | Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461.,Rocha J, Granja AT, Sa-Correia I, Fialho A, Frazao C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt, 1):69-72. Epub 2009 Dec 25. PMID:20057075<ref>PMID:20057075</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Sphingomonas elodea]] | [[Category: Sphingomonas elodea]] | ||
[[Category: UDP-glucose 6-dehydrogenase]] | [[Category: UDP-glucose 6-dehydrogenase]] | ||
| - | [[Category: Fialho, A M | + | [[Category: Fialho, A M]] |
| - | [[Category: Frazao, C | + | [[Category: Frazao, C]] |
| - | [[Category: Granja, A T | + | [[Category: Granja, A T]] |
| - | [[Category: Rocha, J | + | [[Category: Rocha, J]] |
| - | [[Category: Sa-Correia, I | + | [[Category: Sa-Correia, I]] |
[[Category: Carbohydrate synthesis]] | [[Category: Carbohydrate synthesis]] | ||
[[Category: Exopolysaccharide]] | [[Category: Exopolysaccharide]] | ||
[[Category: Gellan]] | [[Category: Gellan]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 14:53, 9 December 2014
Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea
| |||||||||||
