4b7y
From Proteopedia
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| - | [[ | + | ==Crystal structure of the MSL1-MSL2 complex== |
| + | <StructureSection load='4b7y' size='340' side='right' caption='[[4b7y]], [[Resolution|resolution]] 3.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4b7y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Buakea_kaeuae Buakea kaeuae] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B7Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b7y RCSB], [http://www.ebi.ac.uk/pdbsum/4b7y PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Male-Specific Lethal (MSL) complex regulates dosage compensation of the male X chromosome in Drosophila. Here, we report the crystal structure of its MSL1/MSL2 core, where two MSL2 subunits bind to a dimer formed by two molecules of MSL1. Analysis of structure-based mutants revealed that MSL2 can only interact with the MSL1 dimer, but MSL1 dimerization is MSL2 independent. We show that Msl1 is a substrate for Msl2 E3 ubiquitin ligase activity. ChIP experiments revealed that Msl1 dimerization is essential for targeting and spreading of the MSL complex on X-linked genes; however, Msl1 binding to promoters of male and female cells is independent of the dimer status and other MSL proteins. Finally, we show that loss of Msl1 dimerization leads to male-specific lethality. We propose that Msl1-mediated dimerization of the entire MSL complex is required for Msl2 binding, X chromosome recognition, and spreading along the X chromosome. | ||
| - | + | Msl1-mediated dimerization of the dosage compensation complex is essential for male X-chromosome regulation in Drosophila.,Hallacli E, Lipp M, Georgiev P, Spielman C, Cusack S, Akhtar A, Kadlec J Mol Cell. 2012 Nov 30;48(4):587-600. doi: 10.1016/j.molcel.2012.09.014. Epub 2012, Oct 18. PMID:23084835<ref>PMID:23084835</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Buakea kaeuae]] | [[Category: Buakea kaeuae]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Akhtar, A | + | [[Category: Akhtar, A]] |
| - | [[Category: Cusack, S | + | [[Category: Cusack, S]] |
| - | [[Category: Georgiev, P | + | [[Category: Georgiev, P]] |
| - | [[Category: Hallacli, E | + | [[Category: Hallacli, E]] |
| - | [[Category: Kadlec, J | + | [[Category: Kadlec, J]] |
| - | [[Category: Lipp, M | + | [[Category: Lipp, M]] |
| - | [[Category: Spielman, C | + | [[Category: Spielman, C]] |
[[Category: Chromatin]] | [[Category: Chromatin]] | ||
[[Category: Dosage compensation]] | [[Category: Dosage compensation]] | ||
[[Category: Gene regulation]] | [[Category: Gene regulation]] | ||
Revision as of 14:55, 9 December 2014
Crystal structure of the MSL1-MSL2 complex
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