3thk
From Proteopedia
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| - | [[ | + | ==Structure of SH3 chimera with a type II ligand linked to the chain C-terminal== |
| + | <StructureSection load='3thk' size='340' side='right' caption='[[3thk]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3thk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2pqh 2pqh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3THK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3THK FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pwt|1pwt]], [[1shg|1shg]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sptan1, Spna2, Spta2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3thk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3thk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3thk RCSB], [http://www.ebi.ac.uk/pdbsum/3thk PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A new chimeric protein, named WT-CIIA, was designed by connecting the proline-rich decapeptide PPPVPPYSAG to the C-terminus of the alpha-spectrin SH3 domain through a natural twelve-residue linker to obtain a single-chain model that would imitate intramolecular SH3-ligand interaction. The crystal structure of this fusion protein was determined at 1.7 A resolution. The asymmetric unit of the crystal contained two SH3 globules contacting with one PPPVPPY fragment located between them. The domains are related by the two-fold non-crystallographic axis and the ligand lies in two opposite orientations with respect to the conservative binding sites of SH3 domains. | ||
| - | + | High-Resolution Crystal Structure of Spectrin SH3 Domain Fused with a Proline-Rich Peptide.,Gushchina LV, Gabdulkhakov AG, Nikonov SV, Filimonov VV J Biomol Struct Dyn. 2011 Dec;29(3):485-95. PMID:22066535<ref>PMID:22066535</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Spectrin|Spectrin]] | *[[Spectrin|Spectrin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| - | [[Category: Filimonov, V V | + | [[Category: Filimonov, V V]] |
| - | [[Category: Gabdulkhakov, A G | + | [[Category: Gabdulkhakov, A G]] |
| - | [[Category: Gushchina, L V | + | [[Category: Gushchina, L V]] |
| - | [[Category: Nikonov, S V | + | [[Category: Nikonov, S V]] |
| - | [[Category: Nikulin, A D | + | [[Category: Nikulin, A D]] |
[[Category: Chimera]] | [[Category: Chimera]] | ||
[[Category: Sh3 domain]] | [[Category: Sh3 domain]] | ||
[[Category: Structural protein]] | [[Category: Structural protein]] | ||
Revision as of 14:55, 9 December 2014
Structure of SH3 chimera with a type II ligand linked to the chain C-terminal
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