3znh
From Proteopedia
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| - | [[ | + | ==Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.== |
| + | <StructureSection load='3znh' size='340' side='right' caption='[[3znh]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3znh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cchfi Cchfi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZNH FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=AYE:PROP-2-EN-1-AMINE'>AYE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitinyl_hydrolase_1 Ubiquitinyl hydrolase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.12 3.4.19.12] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3znh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3znh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3znh RCSB], [http://www.ebi.ac.uk/pdbsum/3znh PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Active-site directed probes are powerful in studies of enzymatic function. We report an active-site directed probe based on a warhead so far considered unreactive. By replacing the C-terminal carboxylate of ubiquitin (Ub) with an alkyne functionality, a selective reaction with the active-site cysteine residue of de-ubiquitinating enzymes was observed. The resulting product was shown to be a quaternary vinyl thioether, as determined by X-ray crystallography. Proteomic analysis of proteins bound to an immobilized Ub alkyne probe confirmed the selectivity toward de-ubiquitinating enzymes. The observed reactivity is not just restricted to propargylated Ub, as highlighted by the selective reaction between caspase-1 (interleukin converting enzyme) and a propargylated peptide derived from IL-1beta, a caspase-1 substrate. | ||
| - | + | On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases.,Ekkebus R, van Kasteren SI, Kulathu Y, Scholten A, Berlin I, Geurink PP, de Jong A, Goerdayal S, Neefjes J, Heck AJ, Komander D, Ovaa H J Am Chem Soc. 2013 Feb 15. PMID:23387960<ref>PMID:23387960</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Thioesterase|Thioesterase]] | |
| - | == | + | *[[Ubiquitin|Ubiquitin]] |
| - | [[ | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cchfi]] | ||
[[Category: Ubiquitinyl hydrolase 1]] | [[Category: Ubiquitinyl hydrolase 1]] | ||
| - | [[Category: Berlin, I | + | [[Category: Berlin, I]] |
| - | [[Category: Ekkebus, R | + | [[Category: Ekkebus, R]] |
| - | [[Category: Goerdayal, G | + | [[Category: Goerdayal, G]] |
| - | [[Category: Heck, A J.R | + | [[Category: Heck, A J.R]] |
| - | [[Category: Komander, D | + | [[Category: Komander, D]] |
| - | [[Category: Kulathu, Y | + | [[Category: Kulathu, Y]] |
| - | [[Category: Neefjes, J | + | [[Category: Neefjes, J]] |
| - | [[Category: Ovaa, H | + | [[Category: Ovaa, H]] |
| - | [[Category: Scholten, A | + | [[Category: Scholten, A]] |
| - | [[Category: DeJong, A | + | [[Category: DeJong, A]] |
| - | [[Category: VanKasteren, S I | + | [[Category: VanKasteren, S I]] |
[[Category: Deubiquitinase]] | [[Category: Deubiquitinase]] | ||
[[Category: Hydrolase-signaling protein complex]] | [[Category: Hydrolase-signaling protein complex]] | ||
Revision as of 15:04, 9 December 2014
Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.
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