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3u3n

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[[Image:3u3n.png|left|200px]]
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==Crystal structure of tablysin-15==
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<StructureSection load='3u3n' size='340' side='right' caption='[[3u3n]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3u3n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tabanus_yao Tabanus yao]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3N FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u3u|3u3u]], [[3u31|3u31]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3n RCSB], [http://www.ebi.ac.uk/pdbsum/3u3n PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The antihemostatic/antiangiogenic protein tablysin-15 is a member of the cysteine-rich secretory, antigen 5 and pathogenesis-related 1 protein (CAP) superfamily and has been shown to bind the integrins alphaIIbbeta3 and alphaVbeta3 by means of an Arg-Gly-Asp (RGD) tripeptide sequence. Here we describe the X-ray crystal structure of tablysin-15 and show that the RGD motif is located in a novel structural context. The motif itself is contained in a type II beta-turn structure that is similar in its conformation to the RGD sequence of the cyclic pentapeptide cilengitide when bound to integrin alphaVbeta3. The CAP domain also contains a hydrophobic channel that appears to bind a fatty acid molecule in the crystal structure after purification from Escherichia coli. After delipidation of the protein, tablysin-15 was found to bind proinflammatory cysteinyl leukotrienes with submicromolar affinities. The structure of the leukotriene E4 (LTE4)-tablysin-15 complex shows that the ligand binds with the non-functionalized end of the fatty acid chain buried in the hydrophobic pocket, while the carboxylate end of the ligand binds forms hydrogen bond/salt bridge interactions with polar side chains at the channel entrance. Therefore, tablysin-15 functions as an inhibitor of integrin function and as an anti-inflammatory scavenger of eicosanoids.
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{{STRUCTURE_3u3n| PDB=3u3n | SCENE= }}
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Structure of a protein having inhibitory disintegrin and leukotriene scavenging functions contained in a single domain.,Xu X, Francischetti IM, Lai R, Ribeiro JM, Andersen JF J Biol Chem. 2012 Feb 6. PMID:22311975<ref>PMID:22311975</ref>
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===Crystal structure of tablysin-15===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22311975}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3u3n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tabanus_yao Tabanus yao]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3N OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:022311975</ref><references group="xtra"/>
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[[Category: Tabanus yao]]
[[Category: Tabanus yao]]
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[[Category: Andersen, J F.]]
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[[Category: Andersen, J F]]
[[Category: Alphavbeta3 integrin]]
[[Category: Alphavbeta3 integrin]]
[[Category: Binding protein]]
[[Category: Binding protein]]

Revision as of 15:04, 9 December 2014

Crystal structure of tablysin-15

3u3n, resolution 1.65Å

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