1nrv
From Proteopedia
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| - | [[Image:1nrv.gif|left|200px]] | + | [[Image:1nrv.gif|left|200px]] |
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| - | '''Crystal structure of the SH2 domain of Grb10''' | + | {{Structure |
| + | |PDB= 1nrv |SIZE=350|CAPTION= <scene name='initialview01'>1nrv</scene>, resolution 1.65Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= GRB10 OR GRBIR OR KIAA0207 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the SH2 domain of Grb10''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NRV is a [ | + | 1NRV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NRV OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for dimerization of the Grb10 Src homology 2 domain. Implications for ligand specificity., Stein EG, Ghirlando R, Hubbard SR, J Biol Chem. 2003 Apr 11;278(15):13257-64. Epub 2003 Jan 27. PMID:[http:// | + | Structural basis for dimerization of the Grb10 Src homology 2 domain. Implications for ligand specificity., Stein EG, Ghirlando R, Hubbard SR, J Biol Chem. 2003 Apr 11;278(15):13257-64. Epub 2003 Jan 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12551896 12551896] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: dimer]] | [[Category: dimer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:00:51 2008'' |
Revision as of 11:00, 20 March 2008
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| , resolution 1.65Å | |||||||
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| Gene: | GRB10 OR GRBIR OR KIAA0207 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the SH2 domain of Grb10
Overview
Grb7, Grb10, and Grb14 are members of a distinct family of adapter proteins that interact with various receptor tyrosine kinases upon receptor activation. Proteins in this family contain several modular signaling domains including a pleckstrin homology (PH) domain, a BPS (between PH and SH2) domain, and a C-terminal Src homology 2 (SH2) domain. Although SH2 domains are typically monomeric, we show that the Grb10 SH2 domain and also full-length Grb10 gamma are dimeric in solution under physiologic conditions. The crystal structure of the Grb10 SH2 domain at 1.65-A resolution reveals a non-covalent dimer whose interface comprises residues within and flanking the C-terminal alpha helix, which are conserved in the Grb7/Grb10/Grb14 family but not in other SH2 domains. Val-522 in the BG loop (BG3) and Asp-500 in the EF loop (EF1) are positioned to interfere with the binding of the P+3 residue of a phosphopeptide ligand. These structural features of the Grb10 SH2 domain will favor binding of dimeric, turn-containing phosphotyrosine sequences, such as the phosphorylated activation loops in the two beta subunits of the insulin and insulin-like growth factor-1 receptors. Moreover, the structure suggests the mechanism by which the Grb7 SH2 domain binds selectively to pTyr-1139 (pYVNQ) in Her2, which along with Grb7 is co-amplified in human breast cancers.
About this Structure
1NRV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for dimerization of the Grb10 Src homology 2 domain. Implications for ligand specificity., Stein EG, Ghirlando R, Hubbard SR, J Biol Chem. 2003 Apr 11;278(15):13257-64. Epub 2003 Jan 27. PMID:12551896
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