This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1nso
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1nso.gif|left|200px]] | + | [[Image:1nso.gif|left|200px]] |
| - | + | ||
| - | '''Folded monomer of protease from Mason-Pfizer monkey virus''' | + | {{Structure |
| + | |PDB= 1nso |SIZE=350|CAPTION= <scene name='initialview01'>1nso</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= PRT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39068 Simian retrovirus 2]) | ||
| + | }} | ||
| + | |||
| + | '''Folded monomer of protease from Mason-Pfizer monkey virus''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1NSO is a [ | + | 1NSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_retrovirus_2 Simian retrovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSO OCA]. |
==Reference== | ==Reference== | ||
| - | Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:[http:// | + | Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14568536 14568536] |
[[Category: Simian retrovirus 2]] | [[Category: Simian retrovirus 2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: virus maturation]] | [[Category: virus maturation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:01:12 2008'' |
Revision as of 11:01, 20 March 2008
| |||||||
| Gene: | PRT (Simian retrovirus 2) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Folded monomer of protease from Mason-Pfizer monkey virus
Overview
The assembly of Mason-Pfizer monkey virus Gag polyproteins into immature capsids and their cleavage by the encoded protease are temporally and spatially separated processes, making the virus a particularly useful model for investigation of protease activation. Here we present a high resolution NMR structure of a fully folded monomer of a 12 kDa M-PMV protease (wt 12 PR) and of a Cys7Ala/Asp26Asn/Cys106Ala mutant (12 PR(D26N/C7A/C106A)). The overall structures of both wt 12 PR and 12 PR(D26N/C7A/C106A) follow the conservative structural motif of other retroviral proteases. The most prominent difference from the canonical fold of retroviral proteases is the absence of the interfacial beta-sheet, which leads to the loss of the principal force stabilizing the dimer of M-PMV PR. The monomer-dimer equilibrium can be shifted in favor of the dimer by adding a substrate or an inhibitor, partially compensating for the missing role of the beta-sheet. We also show that cysteines C7 and C106 play a crucial role in stabilizing the dimer and consequently increasing the proteolytic activity of M-PMV PR. This is consistent with the role of reversible oxidative modification of the cysteine residues in the regulation of the maturation of assembled M-PMV capsids in the cytoplasm.
About this Structure
1NSO is a Single protein structure of sequence from Simian retrovirus 2. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of a monomeric form of a retroviral protease., Veverka V, Bauerova H, Zabransky A, Lang J, Ruml T, Pichova I, Hrabal R, J Mol Biol. 2003 Oct 31;333(4):771-80. PMID:14568536
Page seeded by OCA on Thu Mar 20 13:01:12 2008
