4fyj

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[[Image:4fyj.jpg|left|200px]]
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==Crystal Structure of P. aeruginosa peptidyl-tRNA hydrolase==
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<StructureSection load='4fyj' size='340' side='right' caption='[[4fyj]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4fyj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYJ FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pth, PA4672 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aminoacyl-tRNA_hydrolase Aminoacyl-tRNA hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.29 3.1.1.29] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyj RCSB], [http://www.ebi.ac.uk/pdbsum/4fyj PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The peptidyl-tRNA hydrolase enzyme from the pathogenic bacterium Pseudomonas aeruginosa (Pth; EC 3.1.1.29) has been cloned, expressed in Escherichia coli and crystallized for X-ray structural analysis. Suitable crystals were grown using the sitting-drop vapour-diffusion method after one week of incubation against a reservoir solution consisting of 20% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10%(v/v) isopropyl alcohol. The crystals were used to obtain the three-dimensional structure of the native protein at 1.77 A resolution. The structure was determined by molecular replacement of the crystallographic data processed in space group P6(1)22 with unit-cell parameters a = b = 63.62, c = 155.20 A, alpha = beta = 90, gamma = 120 degrees . The asymmetric unit of the crystallographic lattice was composed of a single copy of the enzyme molecule with a 43% solvent fraction, corresponding to a Matthews coefficient of 2.43 A(3) Da(-1). The crystallographic structure reported here will serve as the foundation for future structure-guided efforts towards the development of novel small-molecule inhibitors specific to bacterial Pths.
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{{STRUCTURE_4fyj| PDB=4fyj | SCENE= }}
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Recombinant production, crystallization and X-ray crystallographic structure determination of the peptidyl-tRNA hydrolase of Pseudomonas aeruginosa.,Hughes RC, McFeeters H, Coates L, McFeeters RL Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1472-6., doi: 10.1107/S1744309112045770. Epub 2012 Nov 28. PMID:23192026<ref>PMID:23192026</ref>
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===Crystal Structure of P. aeruginosa peptidyl-tRNA hydrolase===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_23192026}}
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==See Also==
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*[[Peptidyl-tRNA hydrolase|Peptidyl-tRNA hydrolase]]
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==About this Structure==
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== References ==
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[[4fyj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYJ OCA].
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aminoacyl-tRNA hydrolase]]
[[Category: Aminoacyl-tRNA hydrolase]]
[[Category: Pseudomonas aeruginosa pao1]]
[[Category: Pseudomonas aeruginosa pao1]]
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[[Category: Hughes, R C.]]
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[[Category: Hughes, R C]]
[[Category: McFeeters, H]]
[[Category: McFeeters, H]]
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[[Category: McFeeters, R L.]]
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[[Category: McFeeters, R L]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 15:45, 9 December 2014

Crystal Structure of P. aeruginosa peptidyl-tRNA hydrolase

4fyj, resolution 1.77Å

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