1nwk
From Proteopedia
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| - | [[Image:1nwk.gif|left|200px]] | + | [[Image:1nwk.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE''' | + | {{Structure |
| + | |PDB= 1nwk |SIZE=350|CAPTION= <scene name='initialview01'>1nwk</scene>, resolution 1.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene> and <scene name='pdbligand=RHO:TETRAMETHYLRHODAMINE-5-MALEIMIDE'>RHO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NWK is a [ | + | 1NWK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWK OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics., Graceffa P, Dominguez R, J Biol Chem. 2003 Sep 5;278(36):34172-80. Epub 2003 Jun 17. PMID:[http:// | + | Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics., Graceffa P, Dominguez R, J Biol Chem. 2003 Sep 5;278(36):34172-80. Epub 2003 Jun 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12813032 12813032] |
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tetramethylrhodamine-5-maleimide]] | [[Category: tetramethylrhodamine-5-maleimide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:02:38 2008'' |
Revision as of 11:02, 20 March 2008
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| , resolution 1.85Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE
Overview
A nucleotide-dependent conformational change regulates actin filament dynamics. Yet, the structural basis of this mechanism remains controversial. The x-ray crystal structure of tetramethylrhodamine-5-maleimide-actin with bound AMPPNP, a non-hydrolyzable ATP analog, was determined to 1.85-A resolution. A comparison of this structure to that of tetramethylrhodamine-5-maleimide-actin with bound ADP, determined previously under similar conditions, reveals how the release of the nucleotide gamma-phosphate sets in motion a sequence of events leading to a conformational change in subdomain 2. The side chain of Ser-14 in the catalytic site rotates upon Pi release, triggering the rearrangement of the loop containing the methylated His-73, referred to as the sensor loop. This in turn causes a transition in the DNase I-binding loop in subdomain 2 from a disordered loop in ATP-actin to an ordered alpha-helix in ADP-actin. Despite this conformational change, the nucleotide cleft remains closed in ADP-actin, similar to ATP-actin. An analysis of the existing structures of members of the actin superfamily suggests that the cleft is open in the nucleotide-free state.
About this Structure
1NWK is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics., Graceffa P, Dominguez R, J Biol Chem. 2003 Sep 5;278(36):34172-80. Epub 2003 Jun 17. PMID:12813032
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