1nx7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1nx7.gif|left|200px]] | + | [[Image:1nx7.gif|left|200px]] |
| - | + | ||
| - | '''Solution Structure of Oxidized Bovine Microsomal Cytochrome B5''' | + | {{Structure |
| + | |PDB= 1nx7 |SIZE=350|CAPTION= <scene name='initialview01'>1nx7</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of Oxidized Bovine Microsomal Cytochrome B5''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1NX7 is a [ | + | 1NX7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX7 OCA]. |
==Reference== | ==Reference== | ||
| - | The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H., Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX, Protein Sci. 2004 Aug;13(8):2161-9. PMID:[http:// | + | The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H., Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX, Protein Sci. 2004 Aug;13(8):2161-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15273310 15273310] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: heme ring]] | [[Category: heme ring]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:02:50 2008'' |
Revision as of 11:02, 20 March 2008
| |||||||
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Oxidized Bovine Microsomal Cytochrome B5
Overview
A comparative study on the solution structures of bovine microsomal cytochrome b5 (Tb5) and the mutant V45H has been achieved by 1D and 2D 1H-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by approximately 3 degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.
About this Structure
1NX7 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H., Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX, Protein Sci. 2004 Aug;13(8):2161-9. PMID:15273310
Page seeded by OCA on Thu Mar 20 13:02:50 2008
Categories: Bos taurus | Single protein | Wu, H. | Zang, Q. | HEM | Five helix | Five sheet | Heme ring
