1nxk

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[[Image:1nxk.gif|left|200px]]<br /><applet load="1nxk" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1nxk.gif|left|200px]]
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caption="1nxk, resolution 2.70&Aring;" />
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'''Crystal structure of staurosporine bound to MAP KAP kinase 2'''<br />
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{{Structure
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|PDB= 1nxk |SIZE=350|CAPTION= <scene name='initialview01'>1nxk</scene>, resolution 2.70&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=STU:STAUROSPORINE'>STU</scene>
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|ACTIVITY=
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|GENE= MAPKAPK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal structure of staurosporine bound to MAP KAP kinase 2'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NXK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=STU:'>STU</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NXK OCA].
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1NXK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NXK OCA].
==Reference==
==Reference==
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Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme., Underwood KW, Parris KD, Federico E, Mosyak L, Czerwinski RM, Shane T, Taylor M, Svenson K, Liu Y, Hsiao CL, Wolfrom S, Maguire M, Malakian K, Telliez JB, Lin LL, Kriz RW, Seehra J, Somers WS, Stahl ML, Structure. 2003 Jun;11(6):627-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12791252 12791252]
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Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme., Underwood KW, Parris KD, Federico E, Mosyak L, Czerwinski RM, Shane T, Taylor M, Svenson K, Liu Y, Hsiao CL, Wolfrom S, Maguire M, Malakian K, Telliez JB, Lin LL, Kriz RW, Seehra J, Somers WS, Stahl ML, Structure. 2003 Jun;11(6):627-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12791252 12791252]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:02:59 2008''

Revision as of 11:03, 20 March 2008

Image:1nxk.gif


PDB ID 1nxk

Drag the structure with the mouse to rotate
, resolution 2.70Å
Ligands: and
Gene: MAPKAPK2 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of staurosporine bound to MAP KAP kinase 2


Overview

MAP KAP kinase 2 (MK2), a Ser/Thr kinase, plays a crucial role in the inflammatory process. We have determined the crystal structures of a catalytically active C-terminal deletion form of human MK2, residues 41-364, in complex with staurosporine at 2.7 A and with ADP at 3.2 A, revealing overall structural similarity with other Ser/Thr kinases. Kinetic analysis reveals that the K(m) for ATP is very similar for MK2 41-364 and p38-activated MK2 41-400. Conversely, the catalytic rate and binding for peptide substrate are dramatically reduced in MK2 41-364. However, phosphorylation of MK2 41-364 by p38 restores the V(max) and K(m) for peptide substrate to values comparable to those seen in p38-activated MK2 41-400, suggesting a mechanism for regulation of enzyme activity.

About this Structure

1NXK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme., Underwood KW, Parris KD, Federico E, Mosyak L, Czerwinski RM, Shane T, Taylor M, Svenson K, Liu Y, Hsiao CL, Wolfrom S, Maguire M, Malakian K, Telliez JB, Lin LL, Kriz RW, Seehra J, Somers WS, Stahl ML, Structure. 2003 Jun;11(6):627-36. PMID:12791252

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