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1nzg

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[[Image:1nzg.gif|left|200px]]<br /><applet load="1nzg" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1nzg.gif|left|200px]]
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caption="1nzg, resolution 1.60&Aring;" />
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'''Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine'''<br />
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{{Structure
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|PDB= 1nzg |SIZE=350|CAPTION= <scene name='initialview01'>1nzg</scene>, resolution 1.60&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NZG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZG OCA].
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1NZG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZG OCA].
==Reference==
==Reference==
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2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] modified oligonucleotides: symbiosis of charge interaction factors and stereoelectronic effects., Prhavc M, Prakash TP, Minasov G, Cook PD, Egli M, Manoharan M, Org Lett. 2003 Jun 12;5(12):2017-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12790517 12790517]
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2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] modified oligonucleotides: symbiosis of charge interaction factors and stereoelectronic effects., Prhavc M, Prakash TP, Minasov G, Cook PD, Egli M, Manoharan M, Org Lett. 2003 Jun 12;5(12):2017-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12790517 12790517]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Egli, M.]]
[[Category: Egli, M.]]
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[[Category: Prakash, T P.]]
[[Category: Prakash, T P.]]
[[Category: Prhavc, M.]]
[[Category: Prhavc, M.]]
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[[Category: 2'-modified oligonucleotides]]
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[[Category: 2'-modified oligonucleotide]]
[[Category: a-dna]]
[[Category: a-dna]]
[[Category: crystal structure]]
[[Category: crystal structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:03:43 2008''

Revision as of 11:03, 20 March 2008


PDB ID 1nzg

Drag the structure with the mouse to rotate
, resolution 1.60Å
Coordinates: save as pdb, mmCIF, xml



Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine


Overview

[structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification have been synthesized. This modification, a cationic analogue of the 2'-O-(2-methoxyethyl) (2'-O-MOE) modification, exhibits high binding affinity to target RNA (but not to DNA) and exceptional resistance to nuclease degradation. Analysis of the crystal structure of a self-complementary oligonucleotide containing a single 2'-O-DMAEOE modification explains the importance of charge factors and gauche effects on the observed antisense properties. 2'-O-DMAEOE modified oligonucleotides are ideal candidates for antisense drugs.

About this Structure

1NZG is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] modified oligonucleotides: symbiosis of charge interaction factors and stereoelectronic effects., Prhavc M, Prakash TP, Minasov G, Cook PD, Egli M, Manoharan M, Org Lett. 2003 Jun 12;5(12):2017-20. PMID:12790517

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