4drw
From Proteopedia
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| - | [[ | + | ==Crystal Structure of the Ternary Complex between S100A10, an Annexin A2 N-terminal Peptide and an AHNAK Peptide== |
| + | <StructureSection load='4drw' size='340' side='right' caption='[[4drw]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4drw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DRW FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S100A10, ANX2LG, CAL1L, CLP11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4drw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4drw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4drw RCSB], [http://www.ebi.ac.uk/pdbsum/4drw PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plasma membrane repair involves the coordinated effort of proteins and the inner phospholipid surface to mend the rupture and return the cell back to homeostasis. Here, we present the three-dimensional structure of a multiprotein complex that includes S100A10, annexin A2, and AHNAK, which along with dysferlin, functions in muscle and cardiac tissue repair. The 3.5 A resolution X-ray structure shows that a single region from the AHNAK C terminus is recruited by an S100A10-annexin A2 heterotetramer, forming an asymmetric ternary complex. The AHNAK peptide adopts a coil conformation that arches across the heterotetramer contacting both annexin A2 and S100A10 protomers with tight affinity ( approximately 30 nM) and establishing a structural rationale whereby both S100A10 and annexin proteins are needed in AHNAK recruitment. The structure evokes a model whereby AHNAK is targeted to the membrane surface through sandwiching of the binding region between the S100A10/annexin A2 complex and the phospholipid membrane. | ||
| - | + | Structure of an asymmetric ternary protein complex provides insight for membrane interaction.,Dempsey BR, Rezvanpour A, Lee TW, Barber KR, Junop MS, Shaw GS Structure. 2012 Oct 10;20(10):1737-45. doi: 10.1016/j.str.2012.08.004. Epub 2012 , Aug 30. PMID:22940583<ref>PMID:22940583</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[S100 protein|S100 protein]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Junop, M S | + | [[Category: Junop, M S]] |
| - | [[Category: Lee, T W | + | [[Category: Lee, T W]] |
| - | [[Category: Rezvanpour, A | + | [[Category: Rezvanpour, A]] |
| - | [[Category: Shaw, G S | + | [[Category: Shaw, G S]] |
[[Category: Atypical ef-hand]] | [[Category: Atypical ef-hand]] | ||
[[Category: Exocytosis-protein binding complex]] | [[Category: Exocytosis-protein binding complex]] | ||
[[Category: Heteropentameric complex]] | [[Category: Heteropentameric complex]] | ||
[[Category: Membrane repair]] | [[Category: Membrane repair]] | ||
Revision as of 16:16, 9 December 2014
Crystal Structure of the Ternary Complex between S100A10, an Annexin A2 N-terminal Peptide and an AHNAK Peptide
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