1nzr
From Proteopedia
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| - | [[Image:1nzr.gif|left|200px]] | + | [[Image:1nzr.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION''' | + | {{Structure |
| + | |PDB= 1nzr |SIZE=350|CAPTION= <scene name='initialview01'>1nzr</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=NO3:NITRATE ION'>NO3</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NZR is a [ | + | 1NZR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZR OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the azurin mutant nickel-Trp48Met from Pseudomonas aeruginosa at 2.2 A resolution., Tsai LC, Sjolin L, Langer V, Bonander N, Karlsson BG, Vanngard T, Hammann C, Nar H, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):711-7. PMID:[http:// | + | Structure of the azurin mutant nickel-Trp48Met from Pseudomonas aeruginosa at 2.2 A resolution., Tsai LC, Sjolin L, Langer V, Bonander N, Karlsson BG, Vanngard T, Hammann C, Nar H, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):711-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299800 15299800] |
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: electron transport (copper)]] | [[Category: electron transport (copper)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:03:52 2008'' |
Revision as of 11:03, 20 March 2008
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| , resolution 2.2Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION
Overview
The structure of the azurin mutant nickel-Trp48Met from Pseudomonas aeruginosa has been determined by difference Fourier synthesis using phases from the wild-type azurin model. The final crystallographic R value is 0.170 for 17 394 reflections to a resolution of 2.2 A. The mutant crystallized in the orthorhombic space group P2(1)2(1)2(1), a = 57.4, b = 80.4, c = 110.3 A. The four molecules in the asymmetric unit are packed as a dimer of dimers. The nickel metal site of this mutant structure is similar to the zinc metal site in the azurin Asp47 mutant. The site-specific mutation was performed at residue Trp48, which is located in the center of the protein in a highly hydrophobic environment, to investigate its suggested role in the long-range electron-transfer pathway between the disulfide bond on one side of the protein to the Cu centre. The structure around the mutation site Met48 showed no significant change compared with the wild-type structure.
About this Structure
1NZR is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Structure of the azurin mutant nickel-Trp48Met from Pseudomonas aeruginosa at 2.2 A resolution., Tsai LC, Sjolin L, Langer V, Bonander N, Karlsson BG, Vanngard T, Hammann C, Nar H, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):711-7. PMID:15299800
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