3vdp
From Proteopedia
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| - | [[ | + | ==Structure and biochemical studies of the recombination mediator protein RecR in RecFOR pathway== |
| + | <StructureSection load='3vdp' size='340' side='right' caption='[[3vdp]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3vdp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis_mb4 Thermoanaerobacter tengcongensis mb4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VDP FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vds|3vds]], [[3vdt|3vdt]], [[3vdu|3vdu]], [[3ve5|3ve5]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">recR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273068 Thermoanaerobacter tengcongensis MB4])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vdp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vdp RCSB], [http://www.ebi.ac.uk/pdbsum/3vdp PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RecR is an important recombination mediator protein in the RecFOR pathway. RecR together with RecO and RecF facilitates RecA nucleoprotein filament formation and homologous pairing. Structural and biochemical studies of Thermoanaerobacter tengcongensis RecR (TTERecR) and its series mutants revealed that TTERecR uses the N-N dimer as a basic functional unit to interact with TTERecO monomer. Two TTERecR N-N dimers form a ring-shaped tetramer via an interaction between their C-terminal regions. The tetramer is a result of crystallization only. Hydrophobic interactions between the entire helix-hairpin-helix domains within the N-terminal regions of two TTERecR monomers are necessary for formation of a RecR functional N-N dimer. The TTERecR N-N dimer conformation also affects formation of a hydrophobic patch, which creates a binding site for TTERecO in the TTERecR Toprim domain. In addition, we demonstrate that TTERecR does not bind single-stranded DNA (ssDNA) and binds double-stranded DNA very weakly, whereas TTERecOR complex can stably bind DNA, with a higher affinity for ssDNA than double-stranded DNA. Based on these results, we propose an interaction model for the RecOR:ssDNA complex. | ||
| - | + | RecOR complex including RecR N-N dimer and RecO monomer displays a high affinity for ssDNA.,Tang Q, Gao P, Liu YP, Gao A, An XM, Liu S, Yan XX, Liang DC Nucleic Acids Res. 2012 Nov 1;40(21):11115-25. doi: 10.1093/nar/gks889. Epub 2012, Sep 27. PMID:23019218<ref>PMID:23019218</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Thermoanaerobacter tengcongensis mb4]] | [[Category: Thermoanaerobacter tengcongensis mb4]] | ||
| - | [[Category: Liang, D C | + | [[Category: Liang, D C]] |
| - | [[Category: Tang, Q | + | [[Category: Tang, Q]] |
| - | [[Category: Yan, X X | + | [[Category: Yan, X X]] |
[[Category: Dna binding]] | [[Category: Dna binding]] | ||
[[Category: Dna repair]] | [[Category: Dna repair]] | ||
[[Category: Recombination]] | [[Category: Recombination]] | ||
[[Category: Zinc finger]] | [[Category: Zinc finger]] | ||
Revision as of 16:21, 9 December 2014
Structure and biochemical studies of the recombination mediator protein RecR in RecFOR pathway
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