4a7e
From Proteopedia
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| - | [[ | + | ==X-ray crystal structure of porcine insulin flash-cooled at high pressure== |
| + | <StructureSection load='4a7e' size='340' side='right' caption='[[4a7e]], [[Resolution|resolution]] 1.86Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4a7e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A7E FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zei|1zei]], [[1b2g|1b2g]], [[3ins|3ins]], [[1wav|1wav]], [[1b2b|1b2b]], [[1b2f|1b2f]], [[1m5a|1m5a]], [[9ins|9ins]], [[1b2d|1b2d]], [[1dei|1dei]], [[1b17|1b17]], [[1sdb|1sdb]], [[1b2a|1b2a]], [[1izb|1izb]], [[2tci|2tci]], [[1b2c|1b2c]], [[4ins|4ins]], [[7ins|7ins]], [[1iza|1iza]], [[3mth|3mth]], [[1b19|1b19]], [[1mpj|1mpj]], [[1zni|1zni]], [[1b2e|1b2e]], [[1b18|1b18]], [[4a7d|4a7d]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4a7e RCSB], [http://www.ebi.ac.uk/pdbsum/4a7e PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | High-pressure freezing (HPF) is a method which allows sample vitrification without cryoprotectants. In the present work, protein crystals were cooled to cryogenic temperatures at a pressure of 210 MPa. In contrast to other HPF methods published to date in the field of cryocrystallography, this protocol involves rapid sample cooling using a standard HPF device. The fast cooling rates allow HPF of protein crystals directly in their mother liquor without the need for cryoprotectants or external reagents. HPF was first attempted with hen egg-white lysozyme and cubic insulin crystals, yielding good to excellent diffraction quality. Non-cryoprotected crystals of the membrane protein photosystem II have been successfully cryocooled for the first time. This indicates that the presented HPF method is well suited to the vitrification of challenging systems with large unit cells and weak crystal contacts. | ||
| - | + | Fast high-pressure freezing of protein crystals in their mother liquor.,Burkhardt A, Warmer M, Panneerselvam S, Wagner A, Zouni A, Glockner C, Reimer R, Hohenberg H, Meents A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):495-500., Epub 2012 Mar 31. PMID:22505429<ref>PMID:22505429</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Molecular Playground/Insulin|Molecular Playground/Insulin]] | *[[Molecular Playground/Insulin|Molecular Playground/Insulin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
| - | [[Category: Burkhardt, A | + | [[Category: Burkhardt, A]] |
| - | [[Category: Hohenberg, H | + | [[Category: Hohenberg, H]] |
| - | [[Category: Meents, A | + | [[Category: Meents, A]] |
| - | [[Category: Panneerselvam, S | + | [[Category: Panneerselvam, S]] |
| - | [[Category: Reimer, R | + | [[Category: Reimer, R]] |
| - | [[Category: Wagner, A | + | [[Category: Wagner, A]] |
| - | [[Category: Warmer, M | + | [[Category: Warmer, M]] |
[[Category: High-pressure cooling]] | [[Category: High-pressure cooling]] | ||
[[Category: High-pressure protein crystallography]] | [[Category: High-pressure protein crystallography]] | ||
[[Category: Hormone]] | [[Category: Hormone]] | ||
Revision as of 17:05, 9 December 2014
X-ray crystal structure of porcine insulin flash-cooled at high pressure
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