3zq4
From Proteopedia
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| - | [[ | + | ==UNUSUAL, DUAL ENDO- AND EXO-NUCLEASE ACTIVITY IN THE DEGRADOSOME EXPLAINED BY CRYSTAL STRUCTURE ANALYSIS OF RNASE J1== |
| + | <StructureSection load='3zq4' size='340' side='right' caption='[[3zq4]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3zq4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQ4 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zq4 RCSB], [http://www.ebi.ac.uk/pdbsum/3zq4 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RNase J is an essential enzyme in Bacillus subtilis with unusual dual endonuclease and 5'-to-3' exonuclease activities that play an important role in the maturation and degradation of mRNA. RNase J is also a component of the recently identified "degradosome" of B. subtilis. We report the crystal structure of RNase J1 from B. subtilis to 3.0 A resolution, analysis of which reveals it to be in an open conformation suitable for binding substrate RNA. RNase J is a member of the beta-CASP family of zinc-dependent metallo-beta-lactamases. We have exploited this similarity in constructing a model for an RNase J1:RNA complex. Analysis of this model reveals candidate-stacking interactions with conserved aromatic side chains, providing a molecular basis for the observed enzyme activity. Comparisons of the B. subtilis RNase J structure with related enzymes reveal key differences that provide insights into conformational changes during catalysis and the role of the C-terminal domain. | ||
| - | + | Unusual, Dual Endo- and Exonuclease Activity in the Degradosome Explained by Crystal Structure Analysis of RNase J1.,Newman JA, Hewitt L, Rodrigues C, Solovyova A, Harwood CR, Lewis RJ Structure. 2011 Sep 7;19(9):1241-51. PMID:21893285<ref>PMID:21893285</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Ribonuclease|Ribonuclease]] | *[[Ribonuclease|Ribonuclease]] | ||
| - | + | *[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]] | |
| - | == | + | == References == |
| - | < | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| - | [[Category: Harwood, C R | + | [[Category: Harwood, C R]] |
| - | [[Category: Hewitt, L | + | [[Category: Hewitt, L]] |
| - | [[Category: Lewis, R J | + | [[Category: Lewis, R J]] |
| - | [[Category: Newman, J A | + | [[Category: Newman, J A]] |
| - | [[Category: Rodrigues, C | + | [[Category: Rodrigues, C]] |
| - | [[Category: Solovyova, A | + | [[Category: Solovyova, A]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Rna maturation]] | [[Category: Rna maturation]] | ||
Revision as of 17:08, 9 December 2014
UNUSUAL, DUAL ENDO- AND EXO-NUCLEASE ACTIVITY IN THE DEGRADOSOME EXPLAINED BY CRYSTAL STRUCTURE ANALYSIS OF RNASE J1
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