4etw
From Proteopedia
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| - | [[ | + | ==Structure of the Enzyme-ACP Substrate Gatekeeper Complex Required for Biotin Synthesis== |
| + | <StructureSection load='4etw' size='340' side='right' caption='[[4etw]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4etw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri] and [http://en.wikipedia.org/wiki/Shigella_flexneri_5_str._8401 Shigella flexneri 5 str. 8401]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ETW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ETW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZMK:METHYL+7-{[2-({N-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-BETA-ALANYL}AMINO)ETHYL]SULFANYL}-7-OXOHEPTANOATE'>ZMK</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bioH, SF3435, S4329 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 Shigella flexneri]), acpP, SFV_1114, SSON_1114 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373384 Shigella flexneri 5 str. 8401])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pimelyl-[acyl-carrier_protein]_methyl_ester_esterase Pimelyl-[acyl-carrier protein] methyl ester esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.85 3.1.1.85] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4etw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4etw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4etw RCSB], [http://www.ebi.ac.uk/pdbsum/4etw PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although the pimeloyl moiety was long known to be a biotin precursor, the mechanism of assembly of this C7 alpha,omega-dicarboxylic acid was only recently elucidated. In Escherichia coli, pimelate is made by bypassing the strict specificity of the fatty acid synthetic pathway. BioC methylates the free carboxyl of a malonyl thioester, which replaces the usual acetyl thioester primer. This atypical primer is transformed to pimeloyl-acyl carrier protein (ACP) methyl ester by two cycles of fatty acid synthesis. The question is, what stops this product from undergoing further elongation? Although BioH readily cleaves this product in vitro, the enzyme is nonspecific, which made assignment of its physiological substrate problematical, especially because another enzyme, BioF, could also perform this gatekeeping function. We report the 2.05-A resolution cocrystal structure of a complex of BioH with pimeloyl-ACP methyl ester and use the structure to demonstrate that BioH is the gatekeeper and its physiological substrate is pimeloyl-ACP methyl ester. | ||
| - | + | Structure of the enzyme-acyl carrier protein (ACP) substrate gatekeeper complex required for biotin synthesis.,Agarwal V, Lin S, Lukk T, Nair SK, Cronan JE Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17406-11. doi:, 10.1073/pnas.1207028109. Epub 2012 Oct 8. PMID:23045647<ref>PMID:23045647</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | == | + | *[[Acyl carrier protein|Acyl carrier protein]] |
| - | [[ | + | *[[Beta-ketoacyl-ACP reductase|Beta-ketoacyl-ACP reductase]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Shigella flexneri]] | [[Category: Shigella flexneri]] | ||
[[Category: Shigella flexneri 5 str. 8401]] | [[Category: Shigella flexneri 5 str. 8401]] | ||
| - | [[Category: Agarwal, V | + | [[Category: Agarwal, V]] |
| - | [[Category: Nair, S K | + | [[Category: Nair, S K]] |
[[Category: Esterase]] | [[Category: Esterase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 17:17, 9 December 2014
Structure of the Enzyme-ACP Substrate Gatekeeper Complex Required for Biotin Synthesis
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