1o87
From Proteopedia
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| - | [[Image:1o87.gif|left|200px]] | + | [[Image:1o87.gif|left|200px]] |
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| - | '''A NEW MGGDP COMPLEX OF THE FFH NG DOMAIN''' | + | {{Structure |
| + | |PDB= 1o87 |SIZE=350|CAPTION= <scene name='initialview01'>1o87</scene>, resolution 2.10Å | ||
| + | |SITE= <scene name='pdbsite=PLA:Mo4+Binding+Site+For+Chain+B'>PLA</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''A NEW MGGDP COMPLEX OF THE FFH NG DOMAIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1O87 is a [ | + | 1O87 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O87 OCA]. |
==Reference== | ==Reference== | ||
| - | Novel protein and Mg2+ configurations in the Mg2+GDP complex of the SRP GTPase ffh., Focia PJ, Alam H, Lu T, Ramirez UD, Freymann DM, Proteins. 2004 Feb 1;54(2):222-30. PMID:[http:// | + | Novel protein and Mg2+ configurations in the Mg2+GDP complex of the SRP GTPase ffh., Focia PJ, Alam H, Lu T, Ramirez UD, Freymann DM, Proteins. 2004 Feb 1;54(2):222-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14696184 14696184] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
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[[Category: srp]] | [[Category: srp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:07:05 2008'' |
Revision as of 11:07, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Sites: | |||||||
| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A NEW MGGDP COMPLEX OF THE FFH NG DOMAIN
Overview
Ffh is the signal sequence recognition and targeting subunit of the prokaryotic signal recognition particle (SRP). Previous structural studies of the NG GTPase domain of Ffh demonstrated magnesium-dependent and magnesium-independent binding conformations for GDP and GMPPNP that are believed to reflect novel mechanisms for exchange and activation in this member of the GTPase superfamily. The current study of the NG GTPase bound to Mg(2+)GDP reveals two new binding conformations-in the first the magnesium interactions are similar to those seen previously, however, the protein undergoes a conformational change that brings a conserved aspartate into its second coordination sphere. In the second, the protein conformation is similar to that seen previously, but the magnesium coordination sphere is disrupted so that only five oxygen ligands are present. The loss of the coordinating water molecule, at the position that would be occupied by the oxygen of the gamma-phosphate of GTP, is consistent with that position being privileged for exchange during phosphate release. The available structures of the GDP-bound protein provide a series of structural snapshots that illuminate steps along the pathway of GDP release following GTP hydrolysis.
About this Structure
1O87 is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Novel protein and Mg2+ configurations in the Mg2+GDP complex of the SRP GTPase ffh., Focia PJ, Alam H, Lu T, Ramirez UD, Freymann DM, Proteins. 2004 Feb 1;54(2):222-30. PMID:14696184
Page seeded by OCA on Thu Mar 20 13:07:05 2008
Categories: Single protein | Thermus aquaticus | Freymann, D M. | CL | FMT | GDP | MG | Ffh | Gtp-binding | Gtpase | Rna-binding | Signal recognition particle | Srp
