3vba
From Proteopedia
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| - | [[ | + | ==Crystal structure of methanogen 3-isopropylmalate isomerase small subunit== |
| + | <StructureSection load='3vba' size='340' side='right' caption='[[3vba]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3vba]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_dsm_2661 Methanocaldococcus jannaschii dsm 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VBA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VBA FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">leuD, MJ1277 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 Methanocaldococcus jannaschii DSM 2661])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vba OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vba RCSB], [http://www.ebi.ac.uk/pdbsum/3vba PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 3-Isopropylmalate/citramalate (IPM) isomerase catalyzes the second step in the leucine biosynthesis pathway. IPM isomerase from Methanococcus jannaschii is a complex protein consisting of a large (MjLeuC) and a small subunit (MjLeuD). It has broad substrate specificity, unlike other bacterial IPM isomerases. In order to understand the reasons for this broad substrate specificity, we determined the crystal structure of MjLeuD at a resolution of 2.0 A. The asymmetric unit contained 6 molecules of LeuD, including three homodimers. The overall structure had a beta/beta/alpha sandwich-fold consisting of 8 alpha-helices and 7 beta-strands. The C-terminal helix, which is important in homodimer formation, showed conformational differences between two homodimer forms of MjLeuD. In addition, we identified a hydrophobic residue (Val28) near the substrate recognition region that may explain the broad substrate specificity of IPM isomerase. Therefore, we suggest that LeuD proteins can be divided into 2 subfamilies, LeuD subfamilies 1 and 2, which show differences in overall structure and in the substrate recognition region. | ||
| - | + | Crystal structure of LeuD from Methanococcus jannaschii.,Lee EH, Cho YW, Hwang KY Biochem Biophys Res Commun. 2012 Mar 9;419(2):160-4. Epub 2012 Feb 9. PMID:22326391<ref>PMID:22326391</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Methanocaldococcus jannaschii dsm 2661]] | [[Category: Methanocaldococcus jannaschii dsm 2661]] | ||
| - | [[Category: Hwang, K Y | + | [[Category: Hwang, K Y]] |
| - | [[Category: Lee, E H | + | [[Category: Lee, E H]] |
[[Category: Cytosol]] | [[Category: Cytosol]] | ||
[[Category: Leud]] | [[Category: Leud]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
Revision as of 17:54, 9 December 2014
Crystal structure of methanogen 3-isopropylmalate isomerase small subunit
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