4gp0
From Proteopedia
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| - | [[ | + | ==The crystal structure of human fascin 1 R149A K150A R151A mutant== |
| + | <StructureSection load='4gp0' size='340' side='right' caption='[[4gp0]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4gp0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GP0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GP0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gp3|4gp3]], [[4gov|4gov]], [[4goy|4goy]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FAN1, FSCN1, HSN, SNL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gp0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gp0 RCSB], [http://www.ebi.ac.uk/pdbsum/4gp0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Filopodia are cell surface protrusions that are essential for cell migration. This finger-like structure is supported by rigid tightly-bundled actin filaments. The protein responsible for actin-bundling in filopodia is fascin. However the mechanism by which fascin functions in filopodial formation is not clear. Here we provide biochemical, cryo-electron tomographic and X-ray crystal structural data demonstrating the unique structural characteristics of fascin. Systematic mutagenesis studies on 100 mutants of fascin indicate that there are two major actin-binding sites on fascin. Crystal structures of four fascin mutants reveal concerted conformational changes in fascin from inactive to active states in the process of actin bundling. Mutations in any one of the actin-binding sites impair the cellular function of fascin in filopodial formation. Altogether, our data reveal the molecular mechanism of fascin function in filopodial formation. | ||
| - | + | Molecular Mechanism of Fascin Function in Filopodial Formation.,Yang S, Huang FK, Huang J, Chen S, Jakoncic J, Leo-Macias A, Diaz-Avalos R, Chen L, Zhang JJ, Huang XY J Biol Chem. 2012 Nov 26. PMID:23184945<ref>PMID:23184945</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Chen, L | + | [[Category: Chen, L]] |
| - | [[Category: Chen, S | + | [[Category: Chen, S]] |
| - | [[Category: Diaz-Avalos, R | + | [[Category: Diaz-Avalos, R]] |
| - | [[Category: Huang, F K | + | [[Category: Huang, F K]] |
| - | [[Category: Huang, J | + | [[Category: Huang, J]] |
| - | [[Category: Huang, X Y | + | [[Category: Huang, X Y]] |
| - | [[Category: Jakoncic, J | + | [[Category: Jakoncic, J]] |
| - | [[Category: Leo-Macias, A | + | [[Category: Leo-Macias, A]] |
| - | [[Category: Yang, S Y | + | [[Category: Yang, S Y]] |
| - | [[Category: Zhang, J J | + | [[Category: Zhang, J J]] |
[[Category: Actin]] | [[Category: Actin]] | ||
[[Category: Actin bundling protein]] | [[Category: Actin bundling protein]] | ||
Revision as of 09:05, 10 December 2014
The crystal structure of human fascin 1 R149A K150A R151A mutant
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Categories: Homo sapiens | Chen, L | Chen, S | Diaz-Avalos, R | Huang, F K | Huang, J | Huang, X Y | Jakoncic, J | Leo-Macias, A | Yang, S Y | Zhang, J J | Actin | Actin bundling protein | Actin-binding | Beta-trefoil | Cancer | Cell migration | Metastasis | Phosphoprotein | Protein binding
