1obu
From Proteopedia
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| - | [[Image:1obu.gif|left|200px]] | + | [[Image:1obu.gif|left|200px]] |
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| - | '''APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY''' | + | {{Structure |
| + | |PDB= 1obu |SIZE=350|CAPTION= <scene name='initialview01'>1obu</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OBU is a [ | + | 1OBU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBU OCA]. |
==Reference== | ==Reference== | ||
| - | Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:[http:// | + | Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12832753 12832753] |
[[Category: Homarus gammarus]] | [[Category: Homarus gammarus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Raftery, J.]] | [[Category: Raftery, J.]] | ||
[[Category: Zagalsky, P F.]] | [[Category: Zagalsky, P F.]] | ||
| - | [[Category: antiparallel beta- | + | [[Category: antiparallel beta-strand]] |
[[Category: lipocalin]] | [[Category: lipocalin]] | ||
[[Category: pigment]] | [[Category: pigment]] | ||
[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:08:37 2008'' |
Revision as of 11:08, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY
Overview
Models of apocrustacyanin C(1) were refined against X-ray data recorded on Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a space-grown and an earth-grown crystal, respectively, both using vapour-diffusion crystal-growth geometry. The space crystals were grown in the APCF on the NASA Space Shuttle. The microgravity crystal growth showed a cyclic nature attributed to Marangoni convection, thus reducing the benefits of the microgravity environment, as reported previously [Chayen et al. (1996), Q. Rev. Biophys. 29, 227-278]. A subsequent mosaicity evaluation, also reported previously, showed only a partial improvement in the space-grown crystals over the earth-grown crystals [Snell et al. (1997), Acta Cryst. D53, 231-239], contrary to the case for lysozyme crystals grown in space with liquid-liquid diffusion, i.e. without any major motion during growth [Snell et al. (1995), Acta Cryst. D52, 1099-1102]. In this paper, apocrustacyanin C(1) electron-density maps from the two refined models are now compared. It is concluded that the electron-density maps of the protein and the bound waters are found to be better overall for the structures of apocrustacyanin C(1) studied from the space-grown crystal compared with those from the earth-grown crystal, even though both crystals were grown using vapour-diffusion crystal-growth geometry. The improved residues are on the surface of the protein, with two involved in or nearby crystal lattice-forming interactions, thus linking an improved crystal-growth mechanism to the molecular level. The structural comparison procedures developed should themselves be valuable for evaluating crystal-growth procedures in the future.
About this Structure
1OBU is a Single protein structure of sequence from Homarus gammarus. Full crystallographic information is available from OCA.
Reference
Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:12832753
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