1ocb

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[[Image:1ocb.gif|left|200px]]<br /><applet load="1ocb" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ocb.gif|left|200px]]
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caption="1ocb, resolution 1.75&Aring;" />
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'''STRUCTURE OF THE WILD-TYPE CELLOBIOHYDROLASE CEL6A FROM HUMICOLAS INSOLENS IN COMPLEX WITH A FLUORESCENT SUBSTRATE'''<br />
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{{Structure
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|PDB= 1ocb |SIZE=350|CAPTION= <scene name='initialview01'>1ocb</scene>, resolution 1.75&Aring;
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|SITE= <scene name='pdbsite=AC1:Flg+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GTM:O1-METHYL-4-DEOXY-4-THIO-BETA-D-GLUCOSE'>GTM</scene>, <scene name='pdbligand=GDA:4-DEOXY-4-AMINO-BETA-D-GLUCOSE'>GDA</scene>, <scene name='pdbligand=FLG:FLUORESCEINYLTHIOUREIDO'>FLG</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]
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|GENE=
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}}
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'''STRUCTURE OF THE WILD-TYPE CELLOBIOHYDROLASE CEL6A FROM HUMICOLAS INSOLENS IN COMPLEX WITH A FLUORESCENT SUBSTRATE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1OCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=GTM:'>GTM</scene>, <scene name='pdbligand=GDA:'>GDA</scene>, <scene name='pdbligand=FLG:'>FLG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] Known structural/functional Site: <scene name='pdbsite=AC1:Flg+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCB OCA].
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1OCB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCB OCA].
==Reference==
==Reference==
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Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens., Varrot A, Frandsen TP, von Ossowski I, Boyer V, Cottaz S, Driguez H, Schulein M, Davies GJ, Structure. 2003 Jul;11(7):855-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12842048 12842048]
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Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens., Varrot A, Frandsen TP, von Ossowski I, Boyer V, Cottaz S, Driguez H, Schulein M, Davies GJ, Structure. 2003 Jul;11(7):855-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12842048 12842048]
[[Category: Cellulose 1,4-beta-cellobiosidase]]
[[Category: Cellulose 1,4-beta-cellobiosidase]]
[[Category: Humicola insolens]]
[[Category: Humicola insolens]]
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[[Category: processive mechanism]]
[[Category: processive mechanism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:08:49 2008''

Revision as of 11:08, 20 March 2008

Image:1ocb.gif


PDB ID 1ocb

Drag the structure with the mouse to rotate
, resolution 1.75Å
Sites:
Ligands: , , , and
Activity: Cellulose 1,4-beta-cellobiosidase, with EC number 3.2.1.91
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE WILD-TYPE CELLOBIOHYDROLASE CEL6A FROM HUMICOLAS INSOLENS IN COMPLEX WITH A FLUORESCENT SUBSTRATE


Overview

The enzymatic digestion of cellulose entails intimate involvement of cellobiohydrolases, whose characteristic active-center tunnel contributes to a processive degradation of the polysaccharide. The cellobiohydrolase Cel6A displays an active site within a tunnel formed by two extended loops, which are known to open and close in response to ligand binding. Here we present five structures of wild-type and mutant forms of Cel6A from Humicola insolens in complex with nonhydrolyzable thio-oligosaccharides, at resolutions from 1.7-1.1 A, dissecting the structural accommodation of a processing substrate chain through the active center during hydrolysis. Movement of ligand is facilitated by extensive solvent-mediated interactions and through flexibility in the hydrophobic surfaces provided by a sheath of tryptophan residues.

About this Structure

1OCB is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.

Reference

Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens., Varrot A, Frandsen TP, von Ossowski I, Boyer V, Cottaz S, Driguez H, Schulein M, Davies GJ, Structure. 2003 Jul;11(7):855-64. PMID:12842048

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