1ofz

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[[Image:1ofz.jpg|left|200px]]<br /><applet load="1ofz" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ofz.jpg|left|200px]]
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caption="1ofz, resolution 1.50&Aring;" />
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'''CRYSTAL STRUCTURE OF FUNGAL LECTIN: SIX-BLADED BETA-PROPELLER FOLD AND NOVEL FUCOSE RECOGNITION MODE FOR ALEURIA AURANTIA LECTIN'''<br />
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{{Structure
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|PDB= 1ofz |SIZE=350|CAPTION= <scene name='initialview01'>1ofz</scene>, resolution 1.50&Aring;
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|SITE= <scene name='pdbsite=AC1:Afl+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene> and <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF FUNGAL LECTIN: SIX-BLADED BETA-PROPELLER FOLD AND NOVEL FUCOSE RECOGNITION MODE FOR ALEURIA AURANTIA LECTIN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1OFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia] with <scene name='pdbligand=FUL:'>FUL</scene> and <scene name='pdbligand=FUC:'>FUC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Afl+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA].
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1OFZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aleuria_aurantia Aleuria aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFZ OCA].
==Reference==
==Reference==
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Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin., Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A, J Biol Chem. 2003 Jul 18;278(29):27059-67. Epub 2003 May 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12732625 12732625]
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Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin., Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A, J Biol Chem. 2003 Jul 18;278(29):27059-67. Epub 2003 May 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12732625 12732625]
[[Category: Aleuria aurantia]]
[[Category: Aleuria aurantia]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: lectin]]
[[Category: lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:10:26 2008''

Revision as of 11:10, 20 March 2008

Image:1ofz.jpg


PDB ID 1ofz

Drag the structure with the mouse to rotate
, resolution 1.50Å
Sites:
Ligands: and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF FUNGAL LECTIN: SIX-BLADED BETA-PROPELLER FOLD AND NOVEL FUCOSE RECOGNITION MODE FOR ALEURIA AURANTIA LECTIN


Overview

Aleuria aurantia lectin is a fungal protein composed of two identical 312-amino acid subunits that specifically recognizes fucosylated glycans. The crystal structure of the lectin complexed with fucose reveals that each monomer consists of a six-bladed beta-propeller fold and of a small antiparallel two-stranded beta-sheet that plays a role in dimerization. Five fucose residues were located in binding pockets between the adjacent propeller blades. Due to repeats in the amino acid sequence, there are strong similarities between the sites. Oxygen atoms O-3, O-4, and O-5 of fucose are involved in hydrogen bonds with side chains of amino acids conserved in all repeats, whereas O-1 and O-2 interact with a large number of water molecules. The nonpolar face of each fucose residue is stacked against the aromatic ring of a Trp or Tyr amino acid, and the methyl group is located in a highly hydrophobic pocket. Depending on the precise binding site geometry, the alpha- or beta-anomer of the fucose ligand is observed bound in the crystal. Surface plasmon resonance experiments conducted on a series of oligosaccharides confirm the broad specificity of the lectin, with a slight preference for alphaFuc1-2Gal disaccharide. This multivalent carbohydrate recognition fold is a new prototype of lectins that is proposed to be involved in the host recognition strategy of several pathogenic organisms including not only the fungi Aspergillus but also the phytopathogenic bacterium Ralstonia solanacearum.

About this Structure

1OFZ is a Single protein structure of sequence from Aleuria aurantia. Full crystallographic information is available from OCA.

Reference

Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin., Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A, J Biol Chem. 2003 Jul 18;278(29):27059-67. Epub 2003 May 5. PMID:12732625

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