1ogv

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[[Image:1ogv.gif|left|200px]]<br /><applet load="1ogv" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ogv.gif|left|200px]]
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caption="1ogv, resolution 2.35&Aring;" />
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'''LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES'''<br />
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{{Structure
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|PDB= 1ogv |SIZE=350|CAPTION= <scene name='initialview01'>1ogv</scene>, resolution 2.35&Aring;
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|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+M'>AC1</scene>
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|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene> and <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1OGV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=BCL:'>BCL</scene>, <scene name='pdbligand=BPH:'>BPH</scene>, <scene name='pdbligand=U10:'>U10</scene> and <scene name='pdbligand=CDL:'>CDL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+M'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA].
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1OGV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA].
==Reference==
==Reference==
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Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12899837 12899837]
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Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12899837 12899837]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Rhodobacter sphaeroides]]
[[Category: Rhodobacter sphaeroides]]
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[[Category: transmembrane]]
[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:10:43 2008''

Revision as of 11:10, 20 March 2008


PDB ID 1ogv

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, resolution 2.35Å
Sites:
Ligands: , , , , and
Coordinates: save as pdb, mmCIF, xml



LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES


Overview

Well-ordered crystals of the bacterial photosynthetic reaction centre from Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we report the type I crystal packing that results from this crystallisation medium, for which 3D crystals grow as stacked 2D crystals, and the reaction centre X-ray structure is refined to 2.35A resolution. In this crystal form, the location of the membrane bilayer could be assigned with confidence. A cardiolipin-binding site is found at the protein-protein interface within the membrane-spanning region, shedding light on the formation of crystal contacts within the membrane. A chloride-binding site was identified in the membrane-spanning region, which suggests a putative site for interaction with the light-harvesting complex I, the cytochrome bc(1) complex or PufX. Comparisons with the X-ray structures of this reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich environment.

About this Structure

1OGV is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:12899837

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