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| - | ==STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A- AND P-SITE TRNAS, AND E-SITE TRNA. THIS FILE CONTAINS THE 50S SUBUNIT FOR MOLECULE I.==
| + | #REDIRECT [[4v5d]] This PDB entry is obsolete and replaced by 4v5d |
| - | <StructureSection load='2wdl' size='340' side='right' caption='[[2wdl]], [[Resolution|resolution]] 3.50Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[2wdl]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WDL FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wdk|2wdk]], [[2wdm|2wdm]], [[2wdn|2wdn]], [[2jl8|2jl8]], [[2b66|2b66]], [[2j03|2j03]], [[1v8q|1v8q]], [[2v47|2v47]], [[2j01|2j01]], [[2jl6|2jl6]], [[1gd8|1gd8]], [[1wki|1wki]], [[2b9p|2b9p]], [[1yl3|1yl3]], [[2b9n|2b9n]], [[2v49|2v49]], [[1giy|1giy]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wdl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wdl RCSB], [http://www.ebi.ac.uk/pdbsum/2wdl PDBsum]</span></td></tr>
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| - | <table>
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| - | == Evolutionary Conservation ==
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| - | [[Image:Consurf_key_small.gif|200px|right]]
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| - | Check<jmol>
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| - | <jmolCheckbox>
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| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/2wdl_consurf.spt"</scriptWhenChecked>
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| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| - | <text>to colour the structure by Evolutionary Conservation</text>
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| - | </jmolCheckbox>
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| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| - | <div style="clear:both"></div>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.
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| - | Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome.,Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482<ref>PMID:19363482</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: 70S Ribosomes]]
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| - | [[Category: RCSB PDB Molecule of the Month]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Kelley, A C.]]
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| - | [[Category: Loakes, D.]]
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| - | [[Category: Ramakrishnan, V.]]
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| - | [[Category: Voorhees, R M.]]
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| - | [[Category: Weixlbaumer, A.]]
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| - | [[Category: Metal-binding]]
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| - | [[Category: Methylation]]
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| - | [[Category: Mrna]]
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| - | [[Category: Paromomycin]]
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| - | [[Category: Repressor]]
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| - | [[Category: Ribonucleoprotein]]
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| - | [[Category: Ribosomal protein]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Rna-binding]]
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| - | [[Category: Rrna-binding]]
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| - | [[Category: Translation]]
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| - | [[Category: Translation regulation]]
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| - | [[Category: Trna]]
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| - | [[Category: Trna-binding]]
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