|
|
| Line 1: |
Line 1: |
| - | ==Promiscuous behavior of proteins in archaeal ribosomes revealed by cryo-EM: implications for evolution of eukaryotic ribosomes (50S ribosomal proteins)==
| + | #REDIRECT [[4v6u]] This PDB entry is obsolete and replaced by 4v6u |
| - | <StructureSection load='3j21' size='340' side='right' caption='[[3j21]], [[Resolution|resolution]] 6.60Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[3j21]] is a 40 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J21 FirstGlance]. <br>
| + | |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j20|3j20]], [[3j2l|3j2l]]</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j21 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j21 RCSB], [http://www.ebi.ac.uk/pdbsum/3j21 PDBsum]</span></td></tr>
| + | |
| - | </table>
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | In all living cells, protein synthesis occurs on ribonucleoprotein particles called ribosomes. Molecular models have been reported for complete bacterial 70S and eukaryotic 80S ribosomes; however, only molecular models of large 50S subunits have been reported for archaea. Here, we present a complete molecular model for the Pyrococcus furiosus 70S ribosome based on a 6.6 A cryo-electron microscopy map. Moreover, we have determined cryo-electron microscopy reconstructions of the Euryarchaeota Methanococcus igneus and Thermococcus kodakaraensis 70S ribosomes and Crenarchaeota Staphylothermus marinus 50S subunit. Examination of these structures reveals a surprising promiscuous behavior of archaeal ribosomal proteins: We observe intersubunit promiscuity of S24e and L8e (L7ae), the latter binding to the head of the small subunit, analogous to S12e in eukaryotes. Moreover, L8e and L14e exhibit intrasubunit promiscuity, being present in two copies per archaeal 50S subunit, with the additional binding site of L14e analogous to the related eukaryotic r-protein L27e. Collectively, these findings suggest insights into the evolution of eukaryotic ribosomal proteins through increased copy number and binding site promiscuity.
| + | |
| - | | + | |
| - | Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution.,Armache JP, Anger AM, Marquez V, Franckenberg S, Frohlich T, Villa E, Berninghausen O, Thomm M, Arnold GJ, Beckmann R, Wilson DN Nucleic Acids Res. 2012 Dec 6. PMID:23222135<ref>PMID:23222135</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | ==See Also==
| + | |
| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Pyrococcus furiosus]]
| + | |
| - | [[Category: Anger, A M]]
| + | |
| - | [[Category: Armache, J P]]
| + | |
| - | [[Category: Arnold, G J]]
| + | |
| - | [[Category: Beckmann, R]]
| + | |
| - | [[Category: Berninghausen, O]]
| + | |
| - | [[Category: Frankenberg, S]]
| + | |
| - | [[Category: Froehlich, T]]
| + | |
| - | [[Category: Marquez, V]]
| + | |
| - | [[Category: Thomm, M]]
| + | |
| - | [[Category: Villa, E]]
| + | |
| - | [[Category: Wilson, D N]]
| + | |
| - | [[Category: Archaea]]
| + | |
| - | [[Category: Archaeal]]
| + | |
| - | [[Category: Kink-turn]]
| + | |
| - | [[Category: Protein synthesis]]
| + | |
| - | [[Category: Ribosomal]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Rna]]
| + | |
