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| - | ==Crystal structure of antibiotic GE82832 bound to 70S ribosome==
| + | #REDIRECT [[4v9s]] This PDB entry is obsolete and replaced by 4v9s |
| - | <StructureSection load='4nw0' size='340' side='right' caption='[[4nw0]], [[Resolution|resolution]] 3.10Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[4nw0]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NW0 FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=004:(2S)-AMINO(PHENYL)ETHANOIC+ACID'>004</scene>, <scene name='pdbligand=2QY:(2Z)-3-(4-HYDROXYPHENYL)-2-(METHYLAMINO)PROP-2-ENOIC+ACID'>2QY</scene>, <scene name='pdbligand=2QZ:N,N-DIMETHYL-L-THREONINE'>2QZ</scene>, <scene name='pdbligand=2R1:(2E)-2-AMINO-4-HYDROXY-3-[(2R)-OXIRAN-2-YL]BUT-2-ENOIC+ACID'>2R1</scene>, <scene name='pdbligand=2R3:(BETAR)-BETA-HYDROXY-O-METHYL-L-TYROSINE'>2R3</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene></td></tr>
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| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nvy|4nvy]], [[4nvz|4nvz]], [[4nw1|4nw1]]</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4nw0 PDBsum]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The translocation of mRNA and tRNA through the ribosome is catalyzed by elongation factor G (EF-G), a universally conserved guanosine triphosphate hydrolase (GTPase). The mechanism by which the closely related decapeptide antibiotics dityromycin and GE82832 inhibit EF-G-catalyzed translocation is elucidated in this study. Using crystallographic and biochemical experiments, we demonstrate that these antibiotics bind to ribosomal protein S12 in solution alone as well as within the small ribosomal subunit, inducing long-range effects on the ribosomal head. The crystal structure of the antibiotic in complex with the 70S ribosome reveals that the binding involves conserved amino acid residues of S12 whose mutations result in in vitro and in vivo antibiotic resistance and loss of antibiotic binding. The data also suggest that GE82832/dityromycin inhibits EF-G-catalyzed translocation by disrupting a critical contact between EF-G and S12 that is required to stabilize the posttranslocational conformation of EF-G, thereby preventing the ribosome-EF-G complex from entering a conformation productive for translocation.
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| - | The antibiotics dityromycin and GE82832 bind protein S12 and block EF-G-catalyzed translocation.,Bulkley D, Brandi L, Polikanov YS, Fabbretti A, O'Connor M, Gualerzi CO, Steitz TA Cell Rep. 2014 Jan 30;6(2):357-65. doi: 10.1016/j.celrep.2013.12.024. Epub 2014, Jan 9. PMID:24412368<ref>PMID:24412368</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Brandi, L.]]
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| - | [[Category: Bulkley, D P.]]
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| - | [[Category: Connor, M O.]]
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| - | [[Category: Fabbretti, A.]]
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| - | [[Category: Gualerzi, C O.]]
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| - | [[Category: Polikanov, Y S.]]
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| - | [[Category: Steitz, T A.]]
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| - | [[Category: 30s subunit with bound mrna]]
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| - | [[Category: 70]]
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| - | [[Category: Dityromycin]]
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| - | [[Category: Dityromycin and ge82832 bind protein s12 and block ef-g catalyzed translocation]]
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| - | [[Category: Ef-g]]
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| - | [[Category: Ge82832]]
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| - | [[Category: P-site trna]]
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| - | [[Category: Ribosomal protein s12]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Ribosome-antibiotic complex]]
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| - | [[Category: Translocation]]
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