|
|
| Line 1: |
Line 1: |
| - | ==mammalian 80S HCV-IRES initiation complex with eIF5B POST-like state==
| + | #REDIRECT [[4ujc]] This PDB entry is obsolete and replaced by 4ujc |
| - | <StructureSection load='4upz' size='340' side='right' caption='[[4upz]], [[Resolution|resolution]] 9.50Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[4upz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UPZ FirstGlance]. <br>
| + | |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uq0|4uq0]], [[4uq1|4uq1]], [[4uq4|4uq4]], [[4upw|4upw]], [[4upx|4upx]], [[4upy|4upy]]</td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4upz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4upz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4upz RCSB], [http://www.ebi.ac.uk/pdbsum/4upz PDBsum]</span></td></tr>
| + | |
| - | <table>
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The universally conserved eukaryotic initiation factor (eIF) 5B, a translational GTPase, is essential for canonical translation initiation. It is also required for initiation facilitated by the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) RNA. eIF5B promotes joining of 60S ribosomal subunits to 40S ribosomal subunits bound by initiator tRNA (Met-tRNAiMet). However, the exact molecular mechanism by which eIF5B acts has not been established. Here we present cryo-EM reconstructions of the mammalian 80S-HCV-IRES-Met-tRNAiMet-eIF5B-GMPPNP complex. We obtained two substates distinguished by the rotational state of the ribosomal subunits and the configuration of initiator tRNA in the peptidyl (P) site. Accordingly, a combination of conformational changes in the 80S ribosome and in initiator tRNA facilitates binding of the Met-tRNAiMet to the 60S P site and redefines the role of eIF5B as a tRNA-reorientation factor.
| + | |
| - | | + | |
| - | Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.,Yamamoto H, Unbehaun A, Loerke J, Behrmann E, Collier M, Burger J, Mielke T, Spahn CM Nat Struct Mol Biol. 2014 Aug;21(8):721-727. doi: 10.1038/nsmb.2859. Epub 2014, Jul 27. PMID:25064512<ref>PMID:25064512</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Hepatitis c virus]]
| + | |
| - | [[Category: Oryctolagus cuniculus]]
| + | |
| - | [[Category: Behrmann, E.]]
| + | |
| - | [[Category: Burger, J.]]
| + | |
| - | [[Category: Loerke, J.]]
| + | |
| - | [[Category: Marianne, C.]]
| + | |
| - | [[Category: Mielke, T.]]
| + | |
| - | [[Category: Spahn, C M.T.]]
| + | |
| - | [[Category: Unbehaun, A.]]
| + | |
| - | [[Category: Yamamoto, H.]]
| + | |
| - | [[Category: Eukaryotic initiation factor 5b]]
| + | |
| - | [[Category: Hepatitis c virus internal ribosome entry site]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Translation initiation]]
| + | |
