1op8
From Proteopedia
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| - | [[Image:1op8.jpg|left|200px]] | + | [[Image:1op8.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of Human Granzyme A''' | + | {{Structure |
| + | |PDB= 1op8 |SIZE=350|CAPTION= <scene name='initialview01'>1op8</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Granzyme_A Granzyme A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.78 3.4.21.78] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Human Granzyme A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OP8 is a [ | + | 1OP8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OP8 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the apoptosis-inducing human granzyme A dimer., Hink-Schauer C, Estebanez-Perpina E, Kurschus FC, Bode W, Jenne DE, Nat Struct Biol. 2003 Jul;10(7):535-40. PMID:[http:// | + | Crystal structure of the apoptosis-inducing human granzyme A dimer., Hink-Schauer C, Estebanez-Perpina E, Kurschus FC, Bode W, Jenne DE, Nat Struct Biol. 2003 Jul;10(7):535-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12819770 12819770] |
[[Category: Granzyme A]] | [[Category: Granzyme A]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
| - | [[Category: granzyme | + | [[Category: granzyme some]] |
[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:13:47 2008'' |
Revision as of 11:13, 20 March 2008
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| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Granzyme A, with EC number 3.4.21.78 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Human Granzyme A
Overview
Granzyme A (GzmA) belongs to a family of trypsin-like serine proteases localized in cytoplasmic granules of activated lymphocytes and natural killer (NK) cells. In contrast to the related granzyme B (GzmB), GzmA forms a stable disulfide-linked homodimer and triggers target-cell death in a caspase-independent way. Limited proteolysis of a high-molecular-mass complex containing SET (also named putative HLA-associated protein II or PHAPII), PHAPI (pp32, leucine-rich acidic nuclear protein) and HMG2 by GzmA liberates NM23-H1, a Mg2+-dependent DNase that causes single-stranded breaks in nuclear DNA. By analyzing the dimeric GzmA structure at a resolution of 2.5 A, we determined the substrate-binding constraints and selective advantages of the two domains arranged as a unique functional tandem. The active sites of the two subunits point in opposite directions and the nearby noncatalytic surfaces can function as exosites, presenting substrates to the active site region of the adjacent partner in a manner analogous to staphylokinase or streptokinase, which present plasminogen to the cofactor-plasmin and cofactor-plasminogen complexes.
About this Structure
1OP8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the apoptosis-inducing human granzyme A dimer., Hink-Schauer C, Estebanez-Perpina E, Kurschus FC, Bode W, Jenne DE, Nat Struct Biol. 2003 Jul;10(7):535-40. PMID:12819770
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