1opc
From Proteopedia
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| - | [[Image:1opc.jpg|left|200px]] | + | [[Image:1opc.jpg|left|200px]] |
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| - | '''OMPR DNA-BINDING DOMAIN, ESCHERICHIA COLI''' | + | {{Structure |
| + | |PDB= 1opc |SIZE=350|CAPTION= <scene name='initialview01'>1opc</scene>, resolution 1.95Å | ||
| + | |SITE= <scene name='pdbsite=ALP:Alpha+Loop,+Site+Of+Interaction+w.+RNA+Polymerase+Alpha+...'>ALP</scene>, <scene name='pdbsite=RH:Recognition+Helix'>RH</scene> and <scene name='pdbsite=W1:Recognition+Wing'>W1</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''OMPR DNA-BINDING DOMAIN, ESCHERICHIA COLI''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OPC is a [ | + | 1OPC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPC OCA]. |
==Reference== | ==Reference== | ||
| - | The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor., Martinez-Hackert E, Stock AM, Structure. 1997 Jan 15;5(1):109-24. PMID:[http:// | + | The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor., Martinez-Hackert E, Stock AM, Structure. 1997 Jan 15;5(1):109-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9016718 9016718] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: winged helix]] | [[Category: winged helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:13:48 2008'' |
Revision as of 11:13, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
OMPR DNA-BINDING DOMAIN, ESCHERICHIA COLI
Overview
BACKGROUND: The differential expression of the ompF and ompC genes is regulated by two proteins that belong to the two component family of signal transduction proteins: the histidine kinase, EnvZ, and the response regulator, OmpR. OmpR belongs to a subfamily of at least 50 response regulators with homologous C-terminal DNA-binding domains of approximately 98 amino acids. Sequence homology with DNA-binding proteins of known structure cannot be detected, and the lack of structural information has prevented understanding of many of this familys functional properties. RESULTS: We have determined the crystal structure of the Escherichia coli OmpR C-terminal domain at 1.95 A resolution. The structure consists of three alpha helices packed against two antiparallel beta sheets. Two helices, alpha2 and alpha3, and the ten residue loop connecting them constitute a variation of the helix-turn-helix (HTH) motif. Helix alpha3 and the loop connecting the two C-terminal beta strands, beta6 and beta7, are probable DNA-recognition sites. Previous mutagenesis studies indicate that the large loop connecting helices alpha2 and alpha3 is the site of interaction with the alpha subunit of RNA polymerase. CONCLUSIONS: OmpRc belongs to the family of 'winged helix-turn-helix' DNA-binding proteins. This relationship, and the results from numerous published mutagenesis studies, have helped us to interpret the functions of most of the structural elements present in this protein domain. The structure of OmpRc could be useful in helping to define the positioning of the alpha subunit of RNA polymerase in relation to transcriptional activators that are bound to DNA.
About this Structure
1OPC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor., Martinez-Hackert E, Stock AM, Structure. 1997 Jan 15;5(1):109-24. PMID:9016718
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