1oql
From Proteopedia
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| - | [[Image:1oql.gif|left|200px]] | + | [[Image:1oql.gif|left|200px]] |
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| - | '''Mistletoe Lectin I from Viscum album complexed with galactose''' | + | {{Structure |
| + | |PDB= 1oql |SIZE=350|CAPTION= <scene name='initialview01'>1oql</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene> and <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Mistletoe Lectin I from Viscum album complexed with galactose''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OQL is a [ | + | 1OQL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQL OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose., Niwa H, Tonevitsky AG, Agapov II, Saward S, Pfuller U, Palmer RA, Eur J Biochem. 2003 Jul;270(13):2739-49. PMID:[http:// | + | Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose., Niwa H, Tonevitsky AG, Agapov II, Saward S, Pfuller U, Palmer RA, Eur J Biochem. 2003 Jul;270(13):2739-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12823544 12823544] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Viscum album]] | [[Category: Viscum album]] | ||
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[[Category: type-ii ribosome-inactivating protein]] | [[Category: type-ii ribosome-inactivating protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:14:17 2008'' |
Revision as of 11:14, 20 March 2008
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| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | rRNA N-glycosylase, with EC number 3.2.2.22 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Mistletoe Lectin I from Viscum album complexed with galactose
Overview
The X-ray structure of mistletoe lectin I (MLI), a type-II ribosome-inactivating protein (RIP), cocrystallized with galactose is described. The model was refined at 3.0 A resolution to an R-factor of 19.9% using 21 899 reflections, with Rfree 24.0%. MLI forms a homodimer (A-B)2 in the crystal, as it does in solution at high concentration. The dimer is formed through contacts between the N-terminal domains of two B-chains involving weak polar and non-polar interactions. Consequently, the overall arrangement of sugar-binding sites in MLI differs from those in monomeric type-II RIPs: two N-terminal sugar-binding sites are 15 A apart on one side of the dimer, and two C-terminal sugar-binding sites are 87 A apart on the other side. Galactose binding is achieved by common hydrogen bonds for the two binding sites via hydroxy groups 3-OH and 4-OH and hydrophobic contact by an aromatic ring. In addition, at the N-terminal site 2-OH forms hydrogen bonds with Asp27 and Lys41, and at the C-terminal site 3-OH and 6-OH undergo water-mediated interactions and C5 has a hydrophobic contact. MLI is a galactose-specific lectin and shows little affinity for N-acetylgalactosamine. The reason for this is discussed. Structural differences among the RIPs investigated in this study (their quaternary structures, location of sugar-binding sites, and fine sugar specificities of their B-chains, which could have diverged through evolution from a two-domain protein) may affect the binding sites, and consequently the cellular transport processes and biological responses of these toxins.
About this Structure
1OQL is a Protein complex structure of sequences from Viscum album. Full crystallographic information is available from OCA.
Reference
Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose., Niwa H, Tonevitsky AG, Agapov II, Saward S, Pfuller U, Palmer RA, Eur J Biochem. 2003 Jul;270(13):2739-49. PMID:12823544
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