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4itr

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[[Image:4itr.jpg|left|200px]]
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==Crystal Structure of IbpAFic2-H3717A in complex with adenylylated Cdc42==
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<StructureSection load='4itr' size='340' side='right' caption='[[4itr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4itr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_somnus_2336 Haemophilus somnus 2336] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3n3v 3n3v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ITR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ITR FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ibpA, p76, HSM_1489 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228400 Haemophilus somnus 2336]), CDC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide-nucleotide_adenylyltransferase Nicotinamide-nucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.1 2.7.7.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4itr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4itr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4itr RCSB], [http://www.ebi.ac.uk/pdbsum/4itr PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Fic family of adenylyltransferases, defined by a core HPFx(D/E)GN(G/K)R motif, consists of over 2,700 proteins found in organisms from bacteria to humans. The immunoglobulin-binding protein A (IbpA) from the bacterial pathogen Histophilus somni contains two Fic domains that adenylylate the switch1 tyrosine residue of Rho-family GTPases, allowing the bacteria to subvert host defenses. Here we present the structure of the second Fic domain of IbpA (IbpAFic2) in complex with its substrate, Cdc42. IbpAFic2-bound Cdc42 mimics the GDI-bound state of Rho GTPases, with both its switch1 and switch2 regions gripped by IbpAFic2. Mutations disrupting the IbpAFic2-Cdc42 interface impair adenylylation and cytotoxicity. Notably, the switch1 tyrosine of Cdc42 is adenylylated in the structure, providing the first structural view for this post-translational modification. We also show that the nucleotide-binding mechanism is conserved among Fic proteins and propose a catalytic mechanism for this recently discovered family of enzymes.
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{{STRUCTURE_4itr| PDB=4itr | SCENE= }}
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Structural basis of Fic-mediated adenylylation.,Xiao J, Worby CA, Mattoo S, Sankaran B, Dixon JE Nat Struct Mol Biol. 2010 Aug;17(8):1004-10. Epub 2010 Jul 11. PMID:20622875<ref>PMID:20622875</ref>
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===Crystal Structure of IbpAFic2-H3717A in complex with adenylylated Cdc42===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20622875}}
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==See Also==
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*[[GTP-binding protein|GTP-binding protein]]
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==About this Structure==
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== References ==
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[[4itr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_somnus_2336 Haemophilus somnus 2336] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3n3v 3n3v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ITR OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:020622875</ref><references group="xtra"/>
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[[Category: Haemophilus somnus 2336]]
[[Category: Haemophilus somnus 2336]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Nicotinamide-nucleotide adenylyltransferase]]
[[Category: Nicotinamide-nucleotide adenylyltransferase]]
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[[Category: Dixon, J E.]]
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[[Category: Dixon, J E]]
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[[Category: Xiao, J.]]
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[[Category: Xiao, J]]
[[Category: Adenosine monophosphate-protein transferase]]
[[Category: Adenosine monophosphate-protein transferase]]
[[Category: Fic domain]]
[[Category: Fic domain]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 16:59, 10 December 2014

Crystal Structure of IbpAFic2-H3717A in complex with adenylylated Cdc42

4itr, resolution 2.30Å

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