1ose

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ose.jpg|left|200px]]<br /><applet load="1ose" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1ose.jpg|left|200px]]
-
caption="1ose, resolution 2.30&Aring;" />
+
 
-
'''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE'''<br />
+
{{Structure
 +
|PDB= 1ose |SIZE=350|CAPTION= <scene name='initialview01'>1ose</scene>, resolution 2.30&Aring;
 +
|SITE= <scene name='pdbsite=AS1:Active+Site,+Sugar+Binding+Site'>AS1</scene>, <scene name='pdbsite=AS2:Active+Site,+Sugar+Binding+Site'>AS2</scene>, <scene name='pdbsite=AS3:Active+Site,+Sugar+Binding+Site'>AS3</scene>, <scene name='pdbsite=AS4:Active+Site,+Sugar+Binding+Site'>AS4</scene>, <scene name='pdbsite=AS5:Active+Site,+Sugar+Binding+Site'>AS5</scene>, <scene name='pdbsite=CAL:Ca+Binding+Site'>CAL</scene> and <scene name='pdbsite=CLO:Chloride+Binding+Site.+There+Are+Six+Bound+Sugar+Rings.+...'>CLO</scene>
 +
|LIGAND= <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]
 +
|GENE=
 +
}}
 +
 
 +
'''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1OSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Sites: <scene name='pdbsite=AS1:Active+Site,+Sugar+Binding+Site'>AS1</scene>, <scene name='pdbsite=AS2:Active+Site,+Sugar+Binding+Site'>AS2</scene>, <scene name='pdbsite=AS3:Active+Site,+Sugar+Binding+Site'>AS3</scene>, <scene name='pdbsite=AS4:Active+Site,+Sugar+Binding+Site'>AS4</scene>, <scene name='pdbsite=AS5:Active+Site,+Sugar+Binding+Site'>AS5</scene>, <scene name='pdbsite=CAL:Ca+Binding+Site'>CAL</scene> and <scene name='pdbsite=CLO:Chloride+Binding+Site.+There+Are+Six+Bound+Sugar+Rings.+...'>CLO</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSE OCA].
+
1OSE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSE OCA].
==Reference==
==Reference==
-
Crystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose., Gilles C, Astier JP, Marchis-Mouren G, Cambillau C, Payan F, Eur J Biochem. 1996 Jun 1;238(2):561-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8681972 8681972]
+
Crystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose., Gilles C, Astier JP, Marchis-Mouren G, Cambillau C, Payan F, Eur J Biochem. 1996 Jun 1;238(2):561-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8681972 8681972]
[[Category: Alpha-amylase]]
[[Category: Alpha-amylase]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 24: Line 33:
[[Category: hydrolase (o-glycosyl)]]
[[Category: hydrolase (o-glycosyl)]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:05 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:14:58 2008''

Revision as of 11:14, 20 March 2008

Image:1ose.jpg


PDB ID 1ose

Drag the structure with the mouse to rotate
, resolution 2.30Å
Sites: , , , , , and
Ligands: , and
Activity: Alpha-amylase, with EC number 3.2.1.1
Coordinates: save as pdb, mmCIF, xml



PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE


Overview

Two different crystal forms of pig pancreatic alpha-amylase isoenzyme II (PPAII), free and complexed to a carbohydrate inhibitor (acarbose), have been compared together and to previously reported structures of PPAI. A crystal form obtained at 4 degrees C, containing nearly 72% solvent, made it possible to obtain a new complex with acarbose, different from a previous one obtained at 20 degrees C [Qian, M., Buisson, G., Duee, E., Haser, H. & Payan, F. (1994) Biochemistry 33, 6284-6294]. In the present form, six contiguous subsites of the enzyme active site are occupied by the carbohydrate ligand; the structural data indicate that the binding site is capable of holding more than the five glucose units of the scheme proposed through kinetic studies. A monosaccharide ring bridging two protein molecules related by the crystal packing is located on the surface, at a distance of 2.0 nm from the reducing end of the inhibitor ligand; the symmetry-related glucose ring in the crystal lattice is found 1.5 nm away from the non-reducing end of the inhibitor ligand.

About this Structure

1OSE is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose., Gilles C, Astier JP, Marchis-Mouren G, Cambillau C, Payan F, Eur J Biochem. 1996 Jun 1;238(2):561-9. PMID:8681972

Page seeded by OCA on Thu Mar 20 13:14:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ose"
Personal tools