1osu

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[[Image:1osu.jpg|left|200px]]<br /><applet load="1osu" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1osu.jpg|left|200px]]
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caption="1osu, resolution 1.400&Aring;" />
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'''STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS'''<br />
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{{Structure
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|PDB= 1osu |SIZE=350|CAPTION= <scene name='initialview01'>1osu</scene>, resolution 1.400&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1OSU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSU OCA].
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1OSU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSU OCA].
==Reference==
==Reference==
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The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs., Wahl MC, Rao ST, Sundaralingam M, Nat Struct Biol. 1996 Jan;3(1):24-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8548450 8548450]
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The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs., Wahl MC, Rao ST, Sundaralingam M, Nat Struct Biol. 1996 Jan;3(1):24-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8548450 8548450]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Rao, S T.]]
[[Category: Rao, S T.]]
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[[Category: nicked]]
[[Category: nicked]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:15:08 2008''

Revision as of 11:15, 20 March 2008


PDB ID 1osu

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, resolution 1.400Å
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS


Overview

The crystal structure of the RNA fragment, 5'-r(UUCGCG)-3', has been determined at 1.4 A resolution by a combination of single isomorphous replacement and molecular search methods. The 3'-terminal CGCG portion of the hexamer engages in Watson-Crick hydrogen bonding while the 5'-terminal UU-overhang forms novel Hoogsteen-like U.U self-base pairs with the overhang of an adjacent duplex. The U.U pairs display a single conventional hydrogen bond between O4 (U1) and N3 (U8) and a CH-O hydrogen bond between C5-H (U1) and O4 (U8), through the Hoogsteen face of the pyrimidine base U1. This unusual arrangement of one of the bases results in a trans U.U pair on antiparallel strands in contrast to the usual cis base pairs. The structure emphasizes the pronounced polymorphism of U.U pairs and has implications for folding of RNA molecules.

About this Structure

1OSU is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

The structure of r(UUCGCG) has a 5'-UU-overhang exhibiting Hoogsteen-like trans U.U base pairs., Wahl MC, Rao ST, Sundaralingam M, Nat Struct Biol. 1996 Jan;3(1):24-31. PMID:8548450

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